Gapmers methods protocols (4 Ergebnisse)

Zustand: New. In English.

Taschenbuch. Zustand: Neu. Druck auf Anfrage Neuware - Printed after ordering - This volume presents a comprehensive collection of detailed state-of-the-art protocols for gapmer-mediated RNA knockdown from leaders in the field. Chapters detail a historical and contemporary perspective of RNase H-inducing antisense oligonucleotides called gapmers and gapmer-mediated RNA knockdown for basic research and therapies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Gapmers: Methods and Protocols aims to provide diverse applications of gapmers along with protocols that will assist readers in moving the frontier.…

Taschenbuch. Zustand: Neu. Gapmers | Methods and Protocols | Toshifumi Yokota (u. a.) | Taschenbuch | xi | Englisch | 2021 | Humana | EAN 9781071607732 | Verantwortliche Person für die EU: Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu. …

Buch. Zustand: Neu. Druck auf Anfrage Neuware - Printed after ordering - This new edition reflects the progression of gapmer research from conceptual foundations to experimental implementation and analytical detection. Beginning with a section covering historical perspectives and the clinical context of antisense gapmers, the book continues with molecular design principles, experimental strategies for developing effective antisense oligonucleotides, methods to evaluate their efficacy and selectivity in cellular and animal models, and analytical approaches for detecting and quantifying antisense oligonucleotides in biological samples. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice needed to ensure success in the lab.Authoritative and up-to-date, Gapmers: Methods and Protocols, Second Edition serves as a valuable resource for researchers and clinicians aiming to accelerate the development of next-generation antisense therapeutics.…