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Hardcover. Zustand: gut. 2010 | 3rd ed. 2011. Fully revised and updated, Flow Cytometry Protocols highlights the expanding contribution of flow cytometry to basic biological research and diagnostic medicine. The text presents cutting-edge topics, such as polychromatic, quantitative, and high throughput flow cytometry, and more. With rapid improvements in instrumentation, lasers, fluorophores, and data analysis software, flow cytometry is riding the crest of unprecedented, innovative advances. This thoroughly revised and up-to-date third edition of Flow Cytometry Protocols highlights the expanding contribution of flow cytometry to basic biological research and diagnostic medicine. Written by leading experts in the field, the book presents cutting-edge topics such as polychromatic, quantitative, and high throughput flow cytometry, novel multiparametric data analysis which breaks the dimensionality barrier, standard practice and safety measures for aerosol-generating cell sorting, conventional and imaging flow cytometry as well as minimalist imaging cytometry. As a volume in the highly successful Methods in Molecular Biology series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and extensive notes on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Flow Cytometry Protocols, Third Edition presents established as well as new flow cytometric methodologies in order to introduce beginning users to basic applications while opening new avenues of innovation for seasoned users. Content: 1. Flow Cytometry: An Introduction Alice L. Givan 2. Breaking the Dimensionality Barrier C. Bruce Bagwell 3. Quantitative Fluorescence Measurements with Multicolor Flow Cytometry Lili Wang, Adolfas K. Gaigalas, and Ming Yan 4. Quantum Dots for Quantitative Flow Cytometry Yang Wu, Larry A. Sklar, and Tione Buranda 5. Bead-Based Multiplexed Analysis of Analytes by Flow Cytometry Henri C. van der Heyde and Irene Gramaglia 6. Flow-Based Combinatorial Antibody Profiling: An Integrated Approach to Cell Characterization Shane Bruckner, Ling Wang, Ruiling Yuan, Perry Haaland, and Amitabh Gaur 7. Tracking Immune Cell Proliferation and Cytotoxic Potential Using Flow Cytometry Joseph D. Tario, Jr., Katharine A. Muirhead, Dalin Pan, Mark E. Munson, and Paul K. Wallace 8. Multiparameter Intracellular Cytokine Staining Patricia Lovelace and Holden T. Maecker 9. Phospho Flow Cytometry Methods for Analysis of Kinase Signaling in Cell Lines and Primary Human Blood Samples Peter O. Krutzik, Angelica Trejo, Kenneth R. Schulz, and Garry P. Nolan 10. Multiparametric Analysis of Apoptosis by Flow Cytometry William G. Telford, Akira Komoriya, Beverly Z. Packard, and C. Bruce Bagwell 11. Multiparameter Cell Cycle Analysis James W. Jacobberger, R. Michael Sramkoski, and Tammy Stefan 12. Rare Event Detection and Analysis in Flow Cytometry: Bone Marrow Mesenchymal Stem Cells, Breast Cancer Stem/Progenitor Cells in Malignant Effusions, and Pericytes in Disaggregated Adipose Tissue Ludovic Zimmerlin, Vera S. Donnenberg, and Albert D. Donnenberg 13. Flow Cytometric-Based Identification of Immature Myeloerythroid Development Cornelis J.H. Pronk and David Bryder 14. Flow Cytometry Immunophenotyping of Hematolymphoid Neoplasia Katherine R. Calvo, Catharine S. McCoy, and Maryalice Stetler-Stevenson 15. Flow Cytometry Assays in Primary Immunodeficiency Diseases Maurice R.G. O'Gorman, Joshua Zollett, and Nicolas Bensen 16. Flow Cytometric Analysis of Microparticles Henri C. van der Heyde, Irene Gramaglia, Valery Combes, Thaddeus C. George, and Georges E. Grau 17. Noncytotoxic DsRed Derivatives for Whole-Cell Labeling Rita L. Strack, Robert J. Keenan, and Benjamin S. Glick 18. Flow Cytometric FRET Analysis of Protein Interaction Gyoergy Vereb, Peter Nagy, and Janos Szoellosi 19. Fluorescent Protein-Assisted Purification for Gene Expression Profiling M. Raza Zaidi, Chi-Ping Day, and Glenn Merlino 20. Multiparametric Analysis, Sorting, and Transcriptional Profiling of Plant Protoplasts and Nuclei According to Cell Type David W. Galbraith, Jaroslav Janda, and Georgina M. Lambert 21. Lentiviral Fluorescent Protein Expression Vectors for Biotinylation Proteomics Irene Riz, Teresa S. Hawley, and Robert G. Hawley 22. Standard Practice for Cell Sorting in a BSL-3 Facility Stephen P. Perfetto, David R. Ambrozak, Richard Nguyen, Mario Roederer, Richard A. Koup, and Kevin L. Holmes 23. The Cytometric Future: It Ain't Necessarily Flow! Howard M. Shapiro Reihe/Serie Methods in Molecular Biology ; 699 Zusatzinfo 26 Tables, black and white; XII, 486 p. Verlagsort Totowa, NJ Sprache englisch Maße 178 x 254 mm Studium 1. Studienabschnitt Vorklinik Histologie Embryologie Studium 2. Studienabschnitt Klinik Anamnese Körperliche Untersuchung Naturwissenschaften Biologie Genetik Molekularbiologie Technik Umwelttechnik Biotechnologie ISBN-10 1-61737-949-2 1617379492 ISBN-13 978-1-61737-949-9 9781617379499 Flow Cytometry Protocols Methods in Molecular Biology Band 699 [Hardcover] Hawley, Teresa S. Hawley, Robert In englischer Sprache. 485 pages. 178 x 254 mm.…

Kartoniert / Broschiert. Zustand: New.

Zustand: New.

Zustand: New. In English.

Zustand: New. In English.

Taschenbuch. Zustand: Neu. Cell and Organ Printing | Bradley R. Ringeisen (u. a.) | Taschenbuch | xiv | Englisch | 2014 | Springer | EAN 9789401785174 | Verantwortliche Person für die EU: Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu. …

Buch. Zustand: Neu. Druck auf Anfrage Neuware - Printed after ordering - Cell and organ printing has become a hot topic of scientific pursuit. Since several early publications between 2000-2003 that demonstrated proof-of-concept, cell and organ printing has blossomed into a rich area for scientific exploration that is being performed by researchers across the globe. Research has thoroughly demonstrated that living cells can be printed via a number of actuations including electrospray, extrusion via micropens and ejection through photothermal, thermal or optical mechanisms. This topic has come of age and it is ripe for exploring the underpinnings of the research to date. We have included research that uses printing technology to deposit or guide cells for tissue engineering applications and for completeness, we have also included chapters describing bacteria printing, biomolecular printing that could be used to build growth factors or recruitment macromolecules into scaffolds, tissue microdissection, as well as live cell printing. The breadth of approaches includes 3D freeform fabrication, ink jet, laser guidance and modified laser direct write techniques. We hope that this book is not the final word but the first word, defining how these tools have been used to take the first steps towards the ultimate goal of creating heterogeneous tissue constructs. Only time will tell whether cell printers will truly become organ printers, but the technologies described in this book hold promise to achieve what the field of regenerative medicine requires - functional 3D scaffolds with multiple cell types differentiated into functional tissue.…

Taschenbuch. Zustand: Neu. Druck auf Anfrage Neuware - Printed after ordering - Cell and organ printing has become a hot topic of scientific pursuit. Since several early publications between 2000-2003 that demonstrated proof-of-concept, cell and organ printing has blossomed into a rich area for scientific exploration that is being performed by researchers across the globe. Research has thoroughly demonstrated that living cells can be printed via a number of actuations including electrospray, extrusion via micropens and ejection through photothermal, thermal or optical mechanisms. This topic has come of age and it is ripe for exploring the underpinnings of the research to date. We have included research that uses printing technology to deposit or guide cells for tissue engineering applications and for completeness, we have also included chapters describing bacteria printing, biomolecular printing that could be used to build growth factors or recruitment macromolecules into scaffolds, tissue microdissection, as well as live cell printing. The breadth of approaches includes 3D freeform fabrication, ink jet, laser guidance and modified laser direct write techniques. We hope that this book is not the final word but the first word, defining how these tools have been used to take the first steps towards the ultimate goal of creating heterogeneous tissue constructs. Only time will tell whether cell printers will truly become organ printers, but the technologies described in this book hold promise to achieve what the field of regenerative medicine requires - functional 3D scaffolds with multiple cell types differentiated into functional tissue.…

Buch. Zustand: Neu. Neuware - Spurred by PRK, LASIK, and other laser-based techniques, refractive surgery is growing at an explosive rate. Within the next decade, laser procedures alone will form a billion-dollar market with more than 500,000 new operations per year.This comprehensive new work-with nearly 300 full-color illustrations-brings the ophthalmologist up-to-date on recent advances in the field, and shows how to use refractive techniques effectively in daily practice. The book examines all major refractive methods, from incisional techniques to laser and lamellar procedures, and in each case provides complete information on patient selection, instrumentation, operative technique, pitfalls and complications, and postoperative management. This is a well-balanced, complete overview of each refractive procedure, thoroughly referenced to maximize usefulness for all ophthalmologists.…