Bovine trypanosomosis in Karamoja region, northeast Uganda: A report of PCR typed trypanosome species and subspecies in Kotido district, northern Karamoja - Softcover

This cross-sectional study provides the first evidence on trypanosome species and subspecies in cattle from Karamoja region, northeast Uganda. In a preliminary survey conducted in May 2011, a total of 706 indigenous cattle from seven communal herds were sampled to detect presence of trypanosomes using micro-haematocrit buffy coat, thick film and thin smear film techniques. The overall herds' prevalence of trypanosomosis was found to be 5.2% (37/706); 11.5% (9/78) in Lokona kraal, 11.0% (15/137) in Lotanyet and 5.9% (13/220) in Natuket, while the other four communal kraals were aparasitaemic. These parasitological results informed second phase sampling design, in which 199 whole blood samples were collected and preserved on FTA® cards for molecular typing. Trypanosoma vivax dominated with a prevalence of 21.1% (42/199) by ITS-PCR, followed by Trypanozoon 15.6% (31/199) by ITS/TBR-PCRs, T. congolense Savannah 1.5% (3/199) and T. simiae 0.5% (1/199). None of the samples amplified for T. godfreyi, and T. congolense Kilifi and Forest subtypes. Only one sample had triple infection with T. vivax, T. congolense Savannah and Trypanozoon, compared to 3.5% (7/199) double infection rate.

Stanley first graduated in 2007, with Bachelor of Veterinary Medicine of Makerere University, and then persued MSc International Animal Health from the University Edinburgh.He currently works in the civil society sector,specializing in epidemiological research, public health and livelihoods food security programming.