This volume presents an outstanding collection of state-of-the-art methodologies for quantifying free radical and antioxidant analytes in tissue and body fluids using experimental models and in vitro procedures. These user-friendly and easily reproducible techniques cover the essential tasks, including radical generating systems, direct measurement or trapping of reactive radical species and acute-phase proteins, and measurement of metabolic intermediates derived from the oxidation of lipids, proteins, and nucleic acids. There are also methods for the determination of vitamin, enzymatic, and water-soluble antioxidants, as well as of essential micronutrients and cofactors. The techniques take advantage of new instrumentation and technology - probes, photon counting, chemiluminescence, and caged compounds, with an emphasis on HPLC - and are adaptable to a wide range of applications. The laboratory-tested free-radical assays described here in detail will illuminate the study of both primary and secondary oxidative stress and contribute significantly to our understanding of the many disorders associated with this process.

Cr�ticas

"...the value of this book lies in the effort by the editor to help collect in one volume useful protocols and methods that will give beginners, as well as veterans in the field, a place to start for study of free radicals and antioxidants." -Analytical Biochemistry "...this is a collection of methods whose acquisition would certainly prove useful to those working in the field of oxidative damage and which should provide plenty of ideas for developments and improvements in research methods in this field."-Angew. Chem. Inst. Ed. "...will be a considerable help to all experimenters, especially those seeking to explore a complex system and identify the species coming into play from the initial stage of oxidants stress, i.e. the generation of the primary ROS, to the formation of secondary radicals and intermediates, the action and regulation of defense systems, and eventually to the termination of the oxidative reaction....I can but highly recommend the book to all - beginners and experienced lab hands alike - who work in the field of oxidative stress, ROS and antioxidants." - Folia Microbiologica

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