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Taschenbuch. Zustand: Neu. Neuware -This detailed volume explores a variety of techniques used to study lineage commitment in stem cells. Further elucidation of the process that stem cells undergo on their way to becoming more specified cell types is vital for a more complete understanding of cell biology and overall physiology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 240 pp. Englisch.

Buch. Zustand: Neu. Neuware -The purpose of this unique title is to provide internal medicine residents and physicians, as well as other professionals engaged in internal medicine practice, with a single resource of comprehensive, abundantly helpful, time-saving training and practice notes. Developed by a now highly experienced hospitalist physician during his three years¿ residency training in internal medicine, as well as during his current role as a practicing hospitalist, these notes provide a broad framework and tool not only for the learning and practicing of internal medicine after graduation from professional schools and during training, but after residency training as well.The majority of the notes were presented as one to a few sentences, rendering the information succinct and easy to digest. The notes also provide simple, key information in patient care including, but not limited to, the workup and management of a wide range of clinical scenarios. The book was divided into three general areas -- 1) daily notes taken during the author¿s residency training (in the format of a diary with the original dates but updated knowledge and information), 2) notes for outpatient medicine and clinical subspecialties, and 3) notes as a hospitalist. The daily notes were based on knowledge and experiences the author learned from actual clinical cases (workup, medication regimen, patient education, and sometimes patient and family interactions). The notes for outpatient medicine and clinical subspecialties were based on specific topics/subspecialties and were heavily clinically oriented with a focus on patient care. The addition of notes as a hospitalist was based on the author¿s duties as a hospitalist, which requires knowledge and understanding of acute neurological and neurosurgical issues, various types of cancers, and some common yet complicated or uncommon clinical scenarios of infectious diseases.A major contribution to the internal medicine education literature, Internal Medicine Training Notes and Survival Guide: An Insider¿s Roadmap for the Journey from Resident to Attending Physician will appeal to a wide readership, including resident physicians, practicing physicians, physician assistants, and nurse practitioners in internal medicine.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 600 pp. Englisch.

Taschenbuch. Zustand: Neu. Neuware -This volume provides methods to study ice-binding proteins (IBPs), and applications involving these proteins. Chapters are divided into three parts describing identifying, isolating, and purifying ice-binding proteins, characterize both IBPs and ice-nucleating proteins (INPs), and synthesize mimics of IBPs, as well as applications involving these proteins. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.Authoritative and cutting-edge, Ice Binding Proteins: Methods and Protocols aims to provide both experimental and simulations protocols to help new researchers, break-the-ice, and enter this exciting field, while also supporting established researchers broaden the scope of theirinvestigations.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 244 pp. Englisch.

Buch. Zustand: Neu. Neuware -This second edition volume expands on the previous edition with updates on the latest methods, resources, and studies concerning analysis and prediction of various structural and functional aspects of proteins and ncRNAs. The chapters in this book cover topics such as secondary structure characterization and prediction; the use and impact of AI (including AlphaFold, large language models, and deep neural networks) in the protein structure prediction field; methods and resources for the prediction of posttranslational modifications, residue-residue contacts, subcellular localization, intrinsic disorder, protein-ligand interactions, and protein aggregation; analysis of cryo-EM data; and analysis of noncoding RNAs in the context of human diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions and surveys of the respective topics, list the necessary materials and methods, cover step-by-step instructions on how to use predictive tools and interpret their results, and provide tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Prediction of Protein Secondary Structure, Second Edition is a valuable resource for anyone interested in understanding the dynamic and growing field of the protein structure prediction.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 368 pp. Englisch.

Buch. Zustand: Neu. Neuware -This volume explores the latest advancements in the high-throughput sequencing (HTS) technologies and its uses in our understanding of complex biological processes and human diseases. The chapters in this book are organized into five parts and cover topics such as genome based-sequencing technologies, including whole genome sequencing, targeted sequencing, and metagenomic analysis; epigenome-based technologies like EM-seq, MNase-seq, and ATAC-seq; transcriptome-based sequencing technologies, including RNA-seq, scRNA-seq, mi-RNA-seq and RIL-seq; HTS modalities for structural studies of DNA including Hi-C and DamID-seq; and the potential use of HTC in clinical settings, including cancer research and treatment, and personalized medicine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, tips on troubleshooting, and avoiding known pitfalls.Cutting-edge and thorough, High Throughput Gene Screening: Methods and Protocols is a valuable resource for all researchers interested in incorporating HTS into their research and learning more about this exciting technology.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 328 pp. Englisch.

Buch. Zustand: Neu. Neuware -This detailed volume presents a comprehensive guide to live-cell RNA imaging techniques and methodologies that have been successfully used to study the dynamics or function of various RNAs in cells. The topics covered encompass techniques including the detection of aptamer-tagged mRNA molecules utilizing fluorogenic proteins, monitoring endogenous mRNA via a split-protein-based probe within neuronal cells, sensitive detection of low-abundance RNAs employing catalytic hairpin assembly circuits, monitoring RNA dynamics using the MS2 system within plant cells, detection of miRNA utilizing DNA-MOF probes, and identification of RNA G-quadruplex structures or nucleolar RNA through fluorescent probes, among other advanced techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Live-Cell RNA Imaging: Methods and Protocols provides researchers with a diverse toolkit for investigating RNA dynamics and function within live cells, thereby contributing to advancements in our understanding of cellular processes at the molecular level.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 220 pp. Englisch.

Buch. Zustand: Neu. Neuware -This volume explores the latest approaches and techniques used to study mitotic exit in diverse model organisms. The chapters in this book cover topics such as transgenic methods generation of RNAi-sensitive cell lines; gene overexpression in heterologue gene expression systems; quantitative live cell imaging and FRET-FLIM; biochemical protocols for analyzing post-translational modifications responsible for mitotic exit regulation; and ways to promote mitosis arrest in disease-associated conditions such as cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and authoritative, Mitotic Exit: Methods and Protocols is a valuable resource for all researchers interested in learning more about this important and advancing field.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 228 pp. Englisch.

Buch. Zustand: Neu. Neuware -This volume aims to be a comprehensive guide to the field of rice molecular biology, encompassing both time-honored techniques and state-of-the-art methodologies. Chapters detail sample preparation, temporal and spatial analysis of RNA, protein expression, transformation and precise genome editing, scalable transcriptome analysis, epigenome analysis, and gene regulatory network analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Rice: Methods and Protocols aims to ensure successful results in the further study of this vital field.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 208 pp. Englisch.

Buch. Zustand: Neu. Neuware -This second volume, details circular economy, innovative materials and techniques, and Omics' techniques to understand the mechanisms and pathways explaining the encapsulation and delivery of the defined nuclei. Chapters will provide sufficient guidance into encapsulation techniques and into the basic understanding of what is needed in terms of tools, materials and supplies to implement innovative approaches in Food Science and Technology. Written in the format of the Methods and Protocols in Food Science (MeFS) series, the chapters include an introduction to the respective topic, list necessary materials and reagents, detail well-established and validated methods for readily reproducible laboratory protocols and contain notes on how to avoid or solve typical problems.Authoritative and cutting-edge, Basic Protocols in Encapsulation of Food Ingredients, Second Edition aims to provide well-established protocols and procedures largely used by both academics and industrials.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 180 pp. Englisch.

Buch. Zustand: Neu. Neuware -This updated volume provides a concise set of protocols on the conceptual principles and practical considerations pertinent to research in mitochondrial function. Driven by the impact of drug- and environmental chemical-induced disturbances on mitochondrial function, hereditary deficiencies, and the age-related decline in bioenergetic performance, this book explores the latest approaches developed in this field by presenting practical principles and assays for quantitative assessments of various parameters reflecting aspects of mitochondrial bioenergetics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, Mitochondrial Bioenergetics: Methods and Protocols, Third Edition serves as an ideal guide for scientists endeavoring to enhance the quality of mitochondrial research.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 312 pp. Englisch.

Buch. Zustand: Neu. Neuware -This second edition volume expands on the previous edition with updates on the latest techniques and technologies used in FOXO research. The chapters in this book are organized into five parts. Part One covers biochemical techniques used to analyze phosphorylation status of FOXO proteins, identify FOXO binding partners, and assess FOXO-specific transcriptional activity. Part Two highlights genetic techniques that are used to identify functional domains and validate disease relevance. Part Three talks about biophysical and bioinformatic methods to study the structure and dynamics of FOXO proteins, and protein-protein interactions. Part Four discusses different fluorescence and imaging techniques to measure FOXO translocation, and Part Five focuses on functional studies in model organisms to characterize cell death regulation and life- and healthspan. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, FOXO Transcription Factors: Methods and Protocols, Second Edition is a valuable resource for both expert and novel researchers who want to learn more about this exciting and developing field of FOXO biology.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 216 pp. Englisch.

Buch. Zustand: Neu. Neuware -This new edition delves into the latest developments in the field and new techniques used to study secondary and supersecondary structures (SSS) in proteins. Beyond the tremendous advances in the field from the AI-based AlphaFold algorithm, researchers continue to untangle how specific structures and protein folds come to be, and these chapters contain numerous techniques to further pursue this study. Written for the highly successful Methods in Molecular Biology series, chapters contain the kind of detailed implementation advice needed to ensure effective results in the lab.Authoritative and practical, Protein Supersecondary Structures: Methods and Protocols, Third Edition serves as a valuable resource for researchers exploring the relationship between amino acids sequences and protein structures, the evolution of proteins, and the dynamics of protein formation.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 416 pp. Englisch.

Buch. Zustand: Neu. Neuware -This volume provides a comprehensive collection of protocols that can be used to study plant chromatin structure and composition. Chapters divided into five sections detail the profiling of chromatin features in relation to epigenetic regulation, investigate the interaction between chromatin modifications and gene regulation, and explore the 3D spatial organization of the chromatin inside the nucleus. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Methods for Plant Nucleus and Chromatin Studies: Methods and Protocols aims to ensure successful results in the further study of this vital field.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 364 pp. Englisch.

Buch. Zustand: Neu. Neuware -This volume aims to introduce the broad field of protocols that can be used for dairy foods and beverages production. Written in the format of the Methods and Protocols in Food Science series, the chapters include an introduction to the respective topic, list necessary materials and reagents, detail well-established and validated methods for readily reproducible laboratory protocols and contain notes on how to avoid or solve typical problems.Authoritative and cutting-edge, Dairy Foods Processing aims to provide sufficient guidance and a basic understanding of the tools, materials, and supplies needed to get started in this important food discipline.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 340 pp. Englisch.

Buch. Zustand: Neu. Neuware -This volume explores the latest advancements in the study of the Marburg virus. The first few chapters in this book review the challenges associated with developing effective medical countermeasures against this virus, while the rest of the chapters provide protocols on ways to achieve these goals. Some of the topics covered in this book include virus isolation and propagation; single-cell transcriptional profiling; reverse genetics systems; proteomics analyses; next-generation sequencing techniques; protocols detailing the use of various animal models including bats and nonhuman primates; and methods for assessing virus stability or validating virus inactivation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Marburg Virus: Methods and Protocols is a valuable resource for all researchers interested in advancing their understanding of this virus.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 472 pp. Englisch.

Buch. Zustand: Neu. Neuware -This detailed volume explores techniques for researching the diverse and specialized mechanisms for mRNA degradation, both in the cytoplasm and the nucleus. From classical methods for studying RNA degradation at the single RNA level to the latest transcriptome-wide approaches involving long-read sequencing and metabolic labeling, this book focuses on methods for eukaryotic models, such as procedures for studying deadenylation, decapping and exoribonuclease activity, assessing RNA decay rate, characterizing RNA degradation intermediates, RNA-proteins interactions, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, mRNA Decay: Methods and Protocols provides both new and experienced RNA researchers with an inspiring collection of protocols to prompt further investigation of these vital degradation pathways.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 440 pp. Englisch.

Buch. Zustand: Neu. Neuware -This second edition details new and updated chapters on technologies related to tertiary lymphoid structures( TLS). Chapters guide readers through TLS in cancers, different stages of TLS development, immune function of TLS, molecular, cellular, and imaging methods, and preclinical methods to uncover the function of cells composing TLS in inflammatory situations in humans. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Tertiary Lymphoid Structures: Methods and Protocols, Second Edition aims to guide you through techniques and methods used to study the biology of TLS.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 328 pp. Englisch.

Buch. Zustand: Neu. Neuware -This detailed volume explores techniques utilizing the fission yeast Schizosaccharomyces pombe, a powerful tractable model system for eukaryotic biology. The book begins with a section on microscopy and imaging, and it continues with chapters covering genome and plasmid editing, including the use of CRISPR/Cas9 technologies, as well as the analysis of DNA damage, repair, and recombination, and more. The volume also includes chapters with a focus on computational methods specific to the approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Schizosaccharomyces pombe: Methods and Protocols serves as the ideal guide for researchers in the fission yeast community working with emerging technologies.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 380 pp. Englisch.