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Taschenbuch. Zustand: Neu. Neuware -This third edition expands on the previous editions with updated and new chapters on protein chromatography. Chapters detail protein stability and storage, avoiding proteolysis, protein quantitation methods, generation and purification of recombinant proteins, recombinant antibody production, and the tagging of proteins. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.Authoritative and cutting-edge, Protein Chromatography: Methods and Protocols, Third Edition aims to provide commonly used methods and new approaches to help both new researchers and experts expand their knowledge.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 508 pp. Englisch.

Taschenbuch. Zustand: Neu. Neuware -This detailed volume explores experimental laboratory procedures for a wide range of steroid bioconversions. After an overview on the current trends and perspectives, the book continues with sections covering microbial screening and synthetic biology applied to microorganisms able to catabolize sterols, methods on strain characterization, including omics and biochemical analyses, methods of fermentation and biocatalysis for steroids production, as well as a chapter on the medical use of glucocorticoids in cancer patients. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 372 pp. Englisch.

Taschenbuch. Zustand: Neu. Neuware -This second edition volume expands on the previous edition with new discussions on the latest techniques used to study molecular chaperones and the stress response. The chapters in this book cover such as analysis of the initiation and regulation of the stress response; the role of heat shock protein 90 (Hsp90) in gene expression through chromosome-immunoprecipitation; features of chaperone function and biology; the emerging role of the extracellular HSPs; and the use of chaperones as biomarkers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 344 pp. Englisch.

Buch. Zustand: Neu. Neuware -This second edition volume expands on the previous edition with updated chapters covering a broader range of tissues and techniques pertaining to stem cell technologies. The chapters also cover topics such as the generation of iPSC-derived cells unique to the individual human genome addressing the possibility of more personalized clinical applications to an individual with a specific degenerative disease; and the use of nanoparticles such as 3D scaffolds and biomaterials as a means of improving stem cell viability after transplantation in the host tissue. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and authoritative, Adult Stem Cells: Methods and Protocols, Second Edition introduces insights into personalized medicine in stem cell therapies, and will spark new and innovative procedures relevant to stem cell therapy and tissue engineering.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 356 pp. Englisch.

Buch. Zustand: Neu. Neuware -This comprehensive guide delves into the protocols and strategies for investigating gene regulation both experimentally and computationally. It focuses on the use of Chromatin Immunoprecipitation (ChIP) coupled with next-generation sequencing, providing a robust framework for profiling DNA-binding proteins, examining DNA footprinting, and analyzing histone modifications. As part of the highly successful Methods in Molecular Biology series, this book offers introductions on the topics covered, detailed lists of the necessary materials and reagents, step-by-step laboratory protocols that are easy to follow, and expert tips for troubleshooting and avoiding known pitfalls.Authoritative and practical, Chromatin Immunoprecipitation: Methods and Protocols is the perfect resource for the new wave of genome biologists looking to master ChIP techniques.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 300 pp. Englisch.

Buch. Zustand: Neu. Neuware -This volume looks at the latest methodological developments used in the field to investigate the synaptic organization of the brain from different viewpoints including structural, structural/functional, molecular, and computational methods. The chapters in this book are organized into five parts and cover topics such as preparation of brain tissue and techniques; connectivity; synaptic vesicles; phenomenological modeling; and dendritic spines. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Authoritative and thorough, New Aspects in Analyzing the Synaptic Organization of the Brain is a valuable resource that will help students, post-docs, and even expert researchers learn more about the discipline of cellular and synaptic neuroscience.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 452 pp. Englisch.

Taschenbuch. Zustand: Neu. Neuware -This second edition details new and updated protocols for experimental approaches that are currently used to study the formation of flowers. Chapters guide readers on genetic methods, phenotypic analyses, genome-wide experiments, modeling, and system-wide approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Flower Development: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 604 pp. Englisch.

Buch. Zustand: Neu. Neuware -This volume details molecular mechanisms that are involved in neuronal morphogenesis, and methods for analyzing these mechanisms. Chapters detail analyzing neuronal morphology in vitro and in vivo using a variety of model systems, including rodents and human induced pluripotent stem cells (hiPSCs). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Neuronal Morphogenesis: Methods and Protocols aims to be suitable for a wide range of readers interested in the mechanisms of neuronal morphology and related neurological disorders.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 400 pp. Englisch.

Buch. Zustand: Neu. Neuware -This volume details protocols on meiosis, covering the latest technological and methodological advances in this research field. Chapters guide readers through methods on genomics, biochemistry, super-resolution microscopy, traditional genetics, cytological methods, as well as machine learning and in silico modelling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Meiosis: Methods and Protocols aims to ensure successful results in further studies of this vital field.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 304 pp. Englisch.

Buch. Zustand: Neu. Neuware -This updated volume presents experimentation-based approaches to lactic acid bacteria (LAB) research. Split into three parts, the book explores techniques for analyzing lactic acid bacteria metabolism and characteristics, applications for food-related industries, such as yogurt production, beer, and wine making, and functions of LAB in human health. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topic, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, Lactic Acid Bacteria: Methods and Protocols, Second Edition serves as an ideal guide for improving research into this vital area of nutrition and health science.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 240 pp. Englisch.

Buch. Zustand: Neu. Neuware Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 260 pp. Englisch.

Buch. Zustand: Neu. Neuware -This volume explores the latest developments in protein secretion research in plants, as compared to yeast and mammalian systems. The chapters in this book present a diverse and thorough perspective of the field and cover topics such as bioinformatic analysis, proteomic studies, ultrastructural analysis, and genetic screening methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Plant Protein Secretion: Methods and Protocols, Second Edition is a valuable resource for researchers and students in the field of plant biology, and will inspire further advancements in our understanding of protein secretion in plant cells and beyond.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 264 pp. Englisch.

Buch. Zustand: Neu. Neuware -This volume details procedures relevant to all disciplines, with specific emphasis on challenging aspects of working with Bacterial Extracellular Vesicle (BEVs). Chapters are divided into four parts focusing on characterization of Bacterial Extracellular Vesicles, assessment of Bacterial Extracellular Vesicle Biological Function In Vitro, preparation and functionalization of therapeutic Bacterial Extracellular Vesicles, and delivery and tracking of Bacterial Extracellular Vesicles In Vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Bacterial Extracellular Vesicles: Methods and Protocols aims to ensure successful results in the further study of this vital field.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 284 pp. Englisch.

Buch. Zustand: Neu. Neuware -This volume provides detailed methods to support fast and reliable diagnosis, surveillance and control of Epizootic Haemorrhagic disease virus (EHDV), with the aim of facilitating and promoting further research into this understudied Orbivirus. Chapters guide readers through methods to enable the characterization of EHDV in vitro and in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Epizootic Hemorrhagic Disease Virus: Methods and Protocols aims to be a useful up-to-date reference work for researchers currently in the field and to those entering the field.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 252 pp. Englisch.

Buch. Zustand: Neu. Neuware -This volume explores the latest experimental and computer-based methods used to study the field of motility research. The chapters in this book cover topics such as methods to investigate motility and chemotaxis in a range of systems including macrophages and Dictyostelium discoideum; ways in which electric fields impact motility and taxis; recently developed computer-based methods for studying pseudopod-driven locomotion of amoeboid cells; and software tools for kymograph-based analysis of cell contour dynamics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and practical, Cell Motility and Chemotaxis: Methods and Protocols is a valuable resource for researchers looking to expand their knowledge of motility-related research across different disciplines.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 236 pp. Englisch.

Buch. Zustand: Neu. Neuware -This volume looks at the latest advancements at the forefront of cellular, circuit, and neural network imaging in the brain. The chapters in this book cover topics such as super resolution imaging at the level of molecules and nanoscopic cellular compartments; the monitoring of individual synapses; physiological communication inside and among neurons and astrocytes; and the activity of targeted multi-cellular ensembles and selected populations of neurons. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Cutting-edge and authoritative, Fluorescence Imaging of the Brain is a valuable resource for both novice and experienced researchers who are interested in learning more about this rapidly evolving area of optical methods and approaches that employ fluorescence sensors to better understand the versatile brain machinery.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 360 pp. Englisch.