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Buch. Zustand: Neu. Neuware - This volume discusses protocols for key laboratory techniques used to study Duchenne and Becker muscular dystrophies. The chapters in this book cover a wide array of topics such as biochemical assays, quantification of dystrophin and related proteins, and measurements of muscle physiology. The chapters also cover methods of DMD sequence analysis; processing and isolation of cells from muscle tissue; culture and study of patient-derived cells; and work with animal models of DMD. Written in the highly successfulMethods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and authoritative, Duchenne Muscular Dystrophy: Methods and Protocols is a valuable resource that will contribute to the standardization of practices in the study of dystrophin and dystrophin-related disease.

Buch. Zustand: Neu. Neuware - This volume focuses on translational models of pediatric brain cancers that are critical to understanding tumor formation, tumor progression, and response to treatment. These models are particularly important for drug screens and preclinical testing of novel treatment approaches involving chemotherapy, radiation, and immunotherapy. The chapters in this book cover a variety of the latest technologies used to investigate the molecular backbone of pediatric brain tumors. These new diagnostic tools include tumor characterization from liquid biopsy; capturing the local heterogeneity of a single tumor through spatial genomics; and deciphering the unique epigenetic traits of pediatric brain tumors by profiling tumor methylome and post-translational histone modifications. Some chapters also describe the latest experimental tools designed to explore new therapeutic strategies and optimize current treatments. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Cutting-edge and thorough, Translational Research Methods for Pediatric Brain Tumors is a valuable resource for clinicians and researchers who want to learn more about experimental pediatric neuro-oncology and help guide the field toward a brighter future.

Buch. Zustand: Neu. Neuware - This second edition details the techniques involved in the biosafety assessment of probiotics to be used for human consumption. New and updated chapters guide readers through identification of antibiotic resistance and virulence genes using various databases, PCR techniques, in vitro cytotoxicity assays, cytolysins, and in vivo toxicity assays such as acute and chronic toxicity, virulence, toxigenicity and dermonecrotic effects. Written in the format of theMethods and Protocols in Food Scienceseries, chapters list necessary materials and methods for readily reproducible protocols.Authoritative and cutting-edge, Biosafety Assessment of Probiotic Potential, Second Eiditionaims to be comprehensive guide for researchers in the field.

Buch. Zustand: Neu. Neuware - This volume details techniques involved in the study of endosymbionts. Chapters divided into four sections detail isolation, maintenance of diverse endosymbionts, classical microscopy, staining, PCR, FISH, flow cytometry, next-generation sequencing, endosymbionts, metabolites, and bacterial endosymbionts in insects. Step-by-step protocols are complemented by personal notes, precautions, and specifying the reagents to be used in each step to ensure the repeatability of the procedure across labs.Authoritative and cutting-edge,Endosymbionts aims to empower researchers to expand the frontiers of endosymbiosis research and unlock new possibilities for science.

Buch. Zustand: Neu. Neuware - This second volume details new and updated methods on biology of various cellular processes and agronomic traits and come up with better strategies to improve legume crops. Chapters guide readers through legume genomic resources, legume pangenome and organelle genome construction, transcriptome analysis, DNA methylation analysis, double-digest restriction site-associated DNA sequencing; target enrichment sequencing via probe capture, genomic selection and transformation methods; prediction of long non-coding RNAs and secondary structures, genome-wide mining of disease resistance gene analogs; genome editing, and bioactive compound and phosphoproteome analysis. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Legume Genomics: Methods and Protocols, Second Edition aims to be comprehensive guide for researchers in the field.

Buch. Zustand: Neu. Neuware - This volume presents a comprehensive collection of protocols and techniques commonly used in research on plant-biotic interactions. Chapters guide readers through general plant pathogen interactions, RNA interference (RNAi) in Okra, spot inoculation of begomoviruses, natural transmission, RNA viruses, enzyme-linked immunosorbent, reverse transcription polymerase chain reaction (RT-PCR), loop-mediated isothermal amplification (RT-LAMP), northern blot hybridization, and next-generation sequencing (NGS) for in-depth analysis of viral populations and mutation patterns. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Plant Biotic Stress: Methods and Protocols aims to be comprehensive guide for researchers in the field.

Buch. Zustand: Neu. Neuware - This book focuses on cutting-edge protocols for novel food analysis, covering advanced techniques for food safety, quality assessment, authentication, and nutritional profiling. It provides step-by-step methodologies for emerging analytical tools such as CRISPR-based detection, nano-sensors, mass spectrometry, lab-on-a-chip technology, spectroscopic techniques, and ultrasonication methods. Written in the format of theMethods and Protocols in Food Scienceseries, chapters list necessary materials and methods for readily reproducible protocols.Authoritative and cutting-edge, Novel Food Analysis: Techniques and Applications aims to be a comprehensive resource for both academic and industrial applications.