springer apr 1992 (9 Ergebnisse)

Taschenbuch. Zustand: Neu. Neuware - Saving Our Planet analyses the changes (both positive and negative) that have occurred in the environment in the past two decades. It focuses not only on the state of the environment but also on the interactions between development activities and the environment. It highlights the main responses since 1972 to protect the environment. It is based on a wide-ranging review of the scientific literatue, UNEP reports (and other sources) and over 500 references are listed.

Buch. Zustand: Neu. Neuware - This book presents case studies of the development of videotex in the UK, France, Germany, Italy, the Netherlands, Austria, Switzerland, Belgium, Denmark, Spain, Sweden, Ireland and the United States. These studies offer an excellent overview of the many factors and actors which might influence the outcome of complex innovation and implementation processes. Videotex is an interlocked innovation comprising not only innovations in the infrastructure (networks, hard- and software) but also an innovation in the supply of services and the user demand. If only one of these elements is absent the entire chain breaks down and the innovation process is hampered. In Europe two different implementation strategies can be distinguished: the Prestel and the Télétel scenarios. Until now, only Télétel has proved successful and only in one country: France. Given this state of affairs the following question is raised -- will the relaunch of videotex succeed in countries where the initial Prestel strategy failed Or will an open market approach, as followed in the United States, present a more direct route to the successful relaunch of videotex in the 1990s This collection presents a coherent up-to-date survey and analysis of videotex systems operating thoughout Europe. It analyses the use, success, limitations and future of videotex systems and related technologies. This collection will be of interest to computer, information and communication scientists, especially those who work in telecommunication and information management. It also offers an excellent case study of information technology policy analysis and as such will be of interest to social and political scientists specialising in technological policy.

Buch. Zustand: Neu. Neuware - 1 The baculoviruses.- 1.1 Introduction.- 1.2 Isolation and host range.- 1.3 Structure and classification.- 1.4 Baculovirus replication in vivo.- 1.5 Baculovirus replication in vitro.- 1.5.1 Baculovirus gene expression and replication.- 1.5.2 Baculovirus gene promoters.- 1.6 Genetic engineering of baculovirus insecticides.- 2 The development of baculovirus expression vectors.- 2.1 Introduction and historical perspective.- 2.2 The merits of the baculovirus expression system.- 2.2.1 Advantages.- 2.2.2 Disadvantages.- 2.3 General principles for inserting foreign genes into the baculovirus genome.- 2.4 Baculovirus transfer vectors.- 2.4.1 Polyhedrin promoter-based expression vectors.- 2.4.2 p10 promoter-based transfer vectors.- 2.4.3 Multiple expression vectors.- 2.4.4 Transfer vectors utilizing other baculovirus gene promoters.- 2.5 Selection of recombinant viruses.- 2.5.1 Selection of a polyhedrin-negative phenotype.- 2.5.2 Selection of f3-galactosidase-negative viruses.- 2.5.3 Recombinant virus selection using dot-blot hybridization.- 2.5.4 Screening for a positive phenotype.- 2.5.5 Enhancing the numbers of recombinant viruses.- 3 Processing of foreign proteins synthesized using baculovirus vectors in insect cells.- 3.1 Introduction.- 3.2 Glycosylation.- 3.3 Phosphorylation, acylation and amidation.- 3.4 Proteolytic processing.- 3.5 Cellular targeting and secretion.- 3.6 Tertiary and quaternary structure formation.- 3.7 Expression of viral genes.- 3.8 Expression of bacterial and fungal genes.- 3.9 Post-transcriptional processing.- 4 Construction of transfer vectors containing the foreign gene.- 4.1 Introduction.- 4.2 Isolation of foreign gene coding sequences.- 4.2.1 Some general guidelines.- 4.2.2 Isolation of DNA fragments from agarose gels.- 4.3 Modifying the ends of DNA molecules.- 4.3.1 Mung bean nuclease.- 4.3.2 Klenow fill-in.- 4.4 Preparation of the transfer vector.- 4.5 DNA ligations.- 4.6 Transformation of bacteria.- 4.7 Screening for recombinant baculovirus transfer vectors.- 4.7.1 Colony hybridization.- 4.7.2 Rapid isolation of bacterial plasmid DNA (mini-preps).- 4.8 Analysis of recombinant transfer vectors.- 4.9 Isolation of highly purified plasmid DNA (maxi-preps).- 5 Insect cell culture media and maintenance of insect cell lines.- 5.1 Introduction.- 5.2 Cell lines.- 5.3 Culture media.- 5.4 Preparation of culture media.- 5.4.1 Preparation of TC100/FCS growth medium.- 5.4.2 Preparation of Grace's (TNM-FH) growth medium.- 5.4.3 Preparation of 4.5 1 TC100 medium from powdered formula.- 5.4.4. Preparation of TC100 medium from individual ingredients.- 5.4.5 Specialized TC100 media.- 5.4.6 Alternative insect cell culture media.- 5.5 Glassware and disposable plasticware.- 5.5.1 Suggested cleaning regime for tissue culture glassware.- 5.6 Insect cell culture.- 5.6.1 Routine sub-culturing of Sf cell lines (monolayer cultures).- 5.6.2 Routine sub-culturing of Sf cells maintained in spinner cultures.- 5.7 A guide to Sf cell seeding densities for experimental work.- 5.8 Freezing, storage and recovery of insect cells in liquid nitrogen.- 5.8.1 Freezing and storage of cells in liquid nitrogen.- 5.8.2 Recovery of cells from liquid nitrogen.- 5.9 A guide to adapting cells to serum-free media.- 6 Propagation, titration and purification of AcMNPV in cell culture.- 6.1 Introduction.- 6.1.1 Safety considerations: general rules for working with baculoviruses.- 6.2 Infection of cells with virus for experimental work.- 6.2.1 Infection of Sf cells in monolayer culture.- 6.2.2 Infection of Sf cells in suspension culture.- 6.3 Titration of virus by plaque-assay.- 6.3.1 Standard plaque-assay.- 6.3.2 Plaque-assay of lacZ-positive viruses.- 6.4 Plaque-picking and plaque-purification.- 6.5 Amplification of virus stocks.- 6.5.1 To prepare a seed stock of virus from a plaque-pick.- 6.5.2 Preparation of an intermediate stock of virus.- 6.5.3 Preparation of a high-titre working stock of virus.- 6.6 Large-scale production of virus for the purifi.

Buch. Zustand: Neu. Neuware - Combination Immunotherapy: Immune Therapy of Human Cancers (R.P. Gale, A. Butturini). Clinical Applications of Combination Chemoimmunotherapy in Cancer: Combination of Fluorouracil and Interfon (R. Pazdur). Combination Approaches and Gene Therapy in the Treatment of AIDS and Other Infectious Diseases: Combination Therapies with Cytokines and AntiCytokines in Murine Opportunistic Infections (L. Romani et al.). Mechanism of Action and Synergy Between Drugs, Biological Response Modifiers and/or Vaccines in Combination Therapies: The Case for Synergy of Thymic Hormones and Interleukins in Immune Reconstitution (J.W. Hadden et al.). Immune Mediated Tumor Destruction (P.M. Sondel). Prospects and Future Directions Using Animal Models to Define Effective Combination Therapies with Biological Response Modifiers: Cancer Immunotherapy (L. Tentori et al.). 29 additional articles. Index.

Buch. Zustand: Neu. Neuware.

Buch. Zustand: Neu. Neuware - Tropical forests affect climate, and the removal of the forests will change climate. Or not This book discusses basic questions on how far, if at all, tropical deforestation leads to climatic change. The question of this uncertainty is particularly addressed. One important consequence of the uncertainties of whether deforestation affects climate is how scientific findings best illuminate the policy-making process.

Buch. Zustand: Neu. Neuware - Why has the energetics of organometallic species become an area of recognized importance What methods are available to obtain information on reaction enthalpies and bond `strengths' What other methods are likely to be used in the future Is the present thermochemical data bank satisfactory for our needs How accurately can we estimate the energetics of organometallic reactions Can theory offer reliable help Which are the main problems to be tackled by the experimentalist These are some of the questions addressed in the present book, which contains contributions by most of the leading groups in the area of organometallic thermochemistry.

Buch. Zustand: Neu. Neuware - Contributed to by leading experts, this book looks at the history of coppice woodlands, their physical environment, the different management techniques used and their effects on the flora and fauna. The implications of this for conservation is controversial and this is debated in a lively way in many of the chapters.

Buch. Zustand: Neu. Neuware - Nitrogen fixation is a key component of the nitrogen cycle, one of the most fundamental cycles in the biosphere. Conversion of atmospheric nitrogen into organic nitrogen compounds can be carried out only by certain bacteria and blue-green algae (cyanobacteria). Some nitrogen fixing bacteria live symbiotically with leguminous plants such as peas, beans, clover, and certain tropical trees in nodules on the plants' roots, while others live independently in the soil and aquatic habitats. By the activity of these microorganisms, the soil is enriched with the nitrogen required for plant growth and function. Thus the topic is of considerable practical as well as fundamental importance. In Biological Nitrogen Fixation, the leading researchers in nitrogen fixation from all over the world contribute up-to-the-minute general reviews on all aspects of the subject, from the molecular biology and genetics to the biochemistry, physiology, and ecology of nitrogen fixation. This compendium of current research is an indispensable reference for all involved in nitrogen fixation research, and of use to all who deal indirectly with the subject. It will also serve as a thoroughly up-to-date textbook for graduate students in microbiology, plant science, biochemistry, molecular biology, plant pathology, agronomy, and genetics.