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Verlag: Springer, Berlin|Springer US|Humana, 2023
ISBN 10: 1071628615 ISBN 13: 9781071628614
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Taschenbuch. Zustand: Neu. The Ubiquitin Code | Manuel S Rodriguez (u. a.) | Taschenbuch | xiii | Englisch | 2023 | Humana | EAN 9781071628614 | Verantwortliche Person für die EU: Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu.
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Taschenbuch. Zustand: Neu. Druck auf Anfrage Neuware - Printed after ordering - This volume details novel and popular approaches to decipher theUbiquitin Code. Chapters guide readers through ubiquitin chain complexity,methods to generate non-hydrolysable ubiquitin-NEDD8,afully synthetic method for the linear synthesis of fluorescently labelled rhodamine LC3A and LC3B,aHECTligase-mediated ubiquitylationin vitro,aRNAi-based screeningto identify DUBs,aYuh1 activityassay,a pull-down to isolateK48 and K63 chainsusingspecific nanobodies,identification of NeDDylated protein based in aNEDD8R74Kmutant,in vivoidentificationof NEDDylated proteins fromliver diseases,UbL-ID method to identifyubiquitin-like modifiedproteins,a LC3/GABARAP capturing system, andmethods to isolate peptides that are closely associated with proteasomes or trapped inside the core particle of proteasomes in eukaryotic cells,among others.Written in the successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,The Ubiquitin Codeaimstoto be auseful practical guide to researches to help further their study in this field.
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Sprache: Englisch
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ISBN 10: 1071628585 ISBN 13: 9781071628584
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Buch. Zustand: Neu. Druck auf Anfrage Neuware - Printed after ordering - This volume details novel and popular approaches to decipher theUbiquitin Code. Chapters guide readers through ubiquitin chain complexity,methods to generate non-hydrolysable ubiquitin-NEDD8,afully synthetic method for the linear synthesis of fluorescently labelled rhodamine LC3A and LC3B,aHECTligase-mediated ubiquitylationin vitro,aRNAi-based screeningto identify DUBs,aYuh1 activityassay,a pull-down to isolateK48 and K63 chainsusingspecific nanobodies,identification of NeDDylated protein based in aNEDD8R74Kmutant,in vivoidentificationof NEDDylated proteins fromliver diseases,UbL-ID method to identifyubiquitin-like modifiedproteins,a LC3/GABARAP capturing system, andmethods to isolate peptides that are closely associated with proteasomes or trapped inside the core particle of proteasomes in eukaryotic cells,among others.Written in the successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,The Ubiquitin Codeaimstoto be auseful practical guide to researches to help further their study in this field.
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In den WarenkorbHardcover. Zustand: Brand New. 258 pages. 10.00x7.01x0.63 inches. In Stock.
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Zustand: New. 2022. 1st ed. 2023. hardcover. . . . . . Books ship from the US and Ireland.