Isbn: 9781493982608 - type 3 secretion systems: methods and protocols (methods in molecular biology, band 1531) (4 Ergebnisse)

Zustand: New. In English.

Taschenbuch. Zustand: Neu. Type 3 Secretion Systems | Methods and Protocols | Matthew L. Nilles (u. a.) | Taschenbuch | x | Englisch | 2018 | Humana | EAN 9781493982608 | Verantwortliche Person für die EU: Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu. …

Taschenbuch. Zustand: Neu. Druck auf Anfrage Neuware - Printed after ordering - This volume discusses various basic and advanced methods and protocols that have been proven to be successful among certain bacterial species, or a family of species, in type III secretion systems (T3S system). The chapters in this book cover topics such as: site-directed mutagenesis and its application in studying the interactions of T3S components; use of transcriptional control to increase expression and secretion of heterologous proteins in T3S systems; fractionation techniques to examine effector translocation; detecting immune responses to T3S systems; mouse immunization with purified needle proteins from T3S systems and the characterization of the immune responses to these proteins; and detection of protein interactions in T3S systems using yeast 2-hybrid analysis. Written in the highly successfulMethods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and comprehensive,Type 3 Secretion Systems: Methods and Protocolsis a valuable resource for anyone interested in learning about the fascinating and ever-changing T3S systems.…

Zustand: New. 2018. Paperback. . . . . . Books ship from the US and Ireland.