ISBN: 9781119394334 - human pluripotent stem cells: a practical guide (4 Ergebnisse)

Human Pluripotent Stem Cells : A Practical Guide
Lakshmipathy, Uma; Macarthur, Chad C.; Sridharan, Mahalakshmi; Quintanilla, Rene H.
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Gebunden. Zustand: New. Über den AutorUma Lakshmipathy, Director R&D, Cell Biology, Life Sciences Solutions, Thermo Fisher Scientific, USA Chad C. MacArthur, Staff Scientist R&D, Cell Biology, Life Sciences Solutions, Thermo Fisher Scie.

- Hardcover
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Buch. Zustand: Neu. Neuware - Comprehensive coverage of the entire induced pluripotent stem cell basic work flow Pluripotent stem cells (PSC) can divide indefinitely, self-renew, and can differentiate to functionally reconstitute almost any cell in the normal developmental pathway, given the right conditions. This comprehensive book, which was developed from a training course, covers all of the PSCs (embryonic, embryonic germ, and embryonic carcinoma) and their functions. It demonstrates the feeder-dependent and feeder-free culture of hESC and hiPSC, which will be referred to in all protocols as PSCs. It also addresses the methods commonly used to determine pluripotency, as defined by self-renewal marker expression and differentiation potential. Human Pluripotent Stem Cells: A Practical Guide offers in-depth chapter coverage of introduction to stem cell, PSC culture, reprogramming, differentiation, PSC characterization, and more. It also includes four appendixes containing information on reagents, medias, and solutions; common antibodies; consumable and equipment; and logs and forms. - Includes helpful tips and tricks that are normally omitted from regular research papers - Features useful images to support the technical aspects and results visually as well as diagrammatic illustrations - Presents specific sections (ie: reprogramming, differentiation) in a concise and easily digestible manner - Written by experts with extensive experience in stem cell technologies Human Pluripotent Stem Cells: A Practical Guide is an ideal text for stem cell researchers, including principal investigators, and others in university and industry settings, and for new graduate students in PSC labs.…