ISBN: 9780443429590 - bacterial efflux systems (volume 724): volume 723 (methods in enzymology, volume 724) (2 Ergebnisse)

Zustand: New. Includes interdisciplinary approaches for analysis of structure-mechanism of the complex nanomachinesProvides approaches to study function in vivoPresents methods for the reconstitution of nanomachines and for measuring activity.

Buch. Zustand: Neu. Neuware - Bacterial Efflux Systems, Volume 722 covers experimental and computational approaches to study the function, structure, and mechanism of bacterial efflux pump systems. The methods covered include preparation of samples for structural and functional analyses and in vivo activity analysis. Specific chapters in this release cover Efflux pump functional assays, NMR and functional analysis efflux pumps, Methods for saturation mutagenesis and functional characterization, Cooperation and functional interplay between pumps, Mapping interactions, HDX-MS, and much more.Other sections cover Recombinant Expression and purification, Reconstitution of a RND efflux pump in different artificial membranes for a structural biology approach, Reconstitution of rnd and mfs efflux pumps for structural biology, CusCBA system, Expression and purification of efflux pumps of Gram positives, Expression and purification of efflux pumps of archaea, ABC transporters: BmrA preparation for NMR and PatA/PatB in peptidisc, In situ structural analysis of efflux assemblies by cryoET, Molecular dynamics simulations, and so much more.…