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Zustand: Used. pp. 173.

Zustand: Used. pp. 173.

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Hardcover. Zustand: gut. 2010 | 3rd ed. 2011. Fully revised and updated, Flow Cytometry Protocols highlights the expanding contribution of flow cytometry to basic biological research and diagnostic medicine. The text presents cutting-edge topics, such as polychromatic, quantitative, and high throughput flow cytometry, and more. With rapid improvements in instrumentation, lasers, fluorophores, and data analysis software, flow cytometry is riding the crest of unprecedented, innovative advances. This thoroughly revised and up-to-date third edition of Flow Cytometry Protocols highlights the expanding contribution of flow cytometry to basic biological research and diagnostic medicine. Written by leading experts in the field, the book presents cutting-edge topics such as polychromatic, quantitative, and high throughput flow cytometry, novel multiparametric data analysis which breaks the dimensionality barrier, standard practice and safety measures for aerosol-generating cell sorting, conventional and imaging flow cytometry as well as minimalist imaging cytometry. As a volume in the highly successful Methods in Molecular Biology series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and extensive notes on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Flow Cytometry Protocols, Third Edition presents established as well as new flow cytometric methodologies in order to introduce beginning users to basic applications while opening new avenues of innovation for seasoned users. Content: 1. Flow Cytometry: An Introduction Alice L. Givan 2. Breaking the Dimensionality Barrier C. Bruce Bagwell 3. Quantitative Fluorescence Measurements with Multicolor Flow Cytometry Lili Wang, Adolfas K. Gaigalas, and Ming Yan 4. Quantum Dots for Quantitative Flow Cytometry Yang Wu, Larry A. Sklar, and Tione Buranda 5. Bead-Based Multiplexed Analysis of Analytes by Flow Cytometry Henri C. van der Heyde and Irene Gramaglia 6. Flow-Based Combinatorial Antibody Profiling: An Integrated Approach to Cell Characterization Shane Bruckner, Ling Wang, Ruiling Yuan, Perry Haaland, and Amitabh Gaur 7. Tracking Immune Cell Proliferation and Cytotoxic Potential Using Flow Cytometry Joseph D. Tario, Jr., Katharine A. Muirhead, Dalin Pan, Mark E. Munson, and Paul K. Wallace 8. Multiparameter Intracellular Cytokine Staining Patricia Lovelace and Holden T. Maecker 9. Phospho Flow Cytometry Methods for Analysis of Kinase Signaling in Cell Lines and Primary Human Blood Samples Peter O. Krutzik, Angelica Trejo, Kenneth R. Schulz, and Garry P. Nolan 10. Multiparametric Analysis of Apoptosis by Flow Cytometry William G. Telford, Akira Komoriya, Beverly Z. Packard, and C. Bruce Bagwell 11. Multiparameter Cell Cycle Analysis James W. Jacobberger, R. Michael Sramkoski, and Tammy Stefan 12. Rare Event Detection and Analysis in Flow Cytometry: Bone Marrow Mesenchymal Stem Cells, Breast Cancer Stem/Progenitor Cells in Malignant Effusions, and Pericytes in Disaggregated Adipose Tissue Ludovic Zimmerlin, Vera S. Donnenberg, and Albert D. Donnenberg 13. Flow Cytometric-Based Identification of Immature Myeloerythroid Development Cornelis J.H. Pronk and David Bryder 14. Flow Cytometry Immunophenotyping of Hematolymphoid Neoplasia Katherine R. Calvo, Catharine S. McCoy, and Maryalice Stetler-Stevenson 15. Flow Cytometry Assays in Primary Immunodeficiency Diseases Maurice R.G. O'Gorman, Joshua Zollett, and Nicolas Bensen 16. Flow Cytometric Analysis of Microparticles Henri C. van der Heyde, Irene Gramaglia, Valery Combes, Thaddeus C. George, and Georges E. Grau 17. Noncytotoxic DsRed Derivatives for Whole-Cell Labeling Rita L. Strack, Robert J. Keenan, and Benjamin S. Glick 18. Flow Cytometric FRET Analysis of Protein Interaction Gyoergy Vereb, Peter Nagy, and Janos Szoellosi 19. Fluorescent Protein-Assisted Purification for Gene Expression Profiling M. Raza Zaidi, Chi-Ping Day, and Glenn Merlino 20. Multiparametric Analysis, Sorting, and Transcriptional Profiling of Plant Protoplasts and Nuclei According to Cell Type David W. Galbraith, Jaroslav Janda, and Georgina M. Lambert 21. Lentiviral Fluorescent Protein Expression Vectors for Biotinylation Proteomics Irene Riz, Teresa S. Hawley, and Robert G. Hawley 22. Standard Practice for Cell Sorting in a BSL-3 Facility Stephen P. Perfetto, David R. Ambrozak, Richard Nguyen, Mario Roederer, Richard A. Koup, and Kevin L. Holmes 23. The Cytometric Future: It Ain't Necessarily Flow! Howard M. Shapiro Reihe/Serie Methods in Molecular Biology ; 699 Zusatzinfo 26 Tables, black and white; XII, 486 p. Verlagsort Totowa, NJ Sprache englisch Maße 178 x 254 mm Studium 1. Studienabschnitt Vorklinik Histologie Embryologie Studium 2. Studienabschnitt Klinik Anamnese Körperliche Untersuchung Naturwissenschaften Biologie Genetik Molekularbiologie Technik Umwelttechnik Biotechnologie ISBN-10 1-61737-949-2 1617379492 ISBN-13 978-1-61737-949-9 9781617379499 Flow Cytometry Protocols Methods in Molecular Biology Band 699 [Hardcover] Hawley, Teresa S. Hawley, Robert In englischer Sprache. 485 pages. 178 x 254 mm.…

Zustand: New.

Paperback. Zustand: Brand New. 186 pages. 9.25x6.10x0.40 inches. In Stock.

Taschenbuch. Zustand: Neu. Druck auf Anfrage Neuware - Printed after ordering - At the conclusion of the Conference on Tribology in the area of Wear Life Pre diction of Mechanical Components, which was held at the General Motors Research Laboratories and sponsored by the Industrial Research Institute, a very high pri ority recommendation was modeling of tribological systems. Since the appearance of the Conference Proceedings in 1985, the writers discussed the matter of modeling with Dr. Edward A. Saibel, Chief of the Solid Mechanics Branch, Engineering Sciences Division, U.S. Army Research Office. This discussion led to a proposal for the Workshop which resulted in this volume. The choice of proposal and Workshop name turned out to be more restricted than it needed to be. As such, the Workshop adopted the name for this volume, Approaches to Modeling of Friction and Wear. By design, the attendance was restricted to not more than 40 individuals so as to allow small group discussions. There were four panels which deliberated on the same questions after two invited area lectures. Section 1 contains the substance of the two lectures. Section 2 is the Workshop Summary which is a distillation of the four panel reports by the entire Workshop attendance. This was formally written up and edited by the eight panel session chairmen, i.e., each of the four panels met twice on two different questions under the leadership of a chairman for each session. Section 3 contains four brief position papers on the subject of the Workshop.…