chang thomas ming swi (44 Ergebnisse)

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Taschenbuch. Zustand: Neu. Druck auf Anfrage Neuware - Printed after ordering - I) ADSORPTION EEEEEEEE E E carrier 2) COVALENT LINKAGE a) Insoluble support b) Intermolecular linkage N'E~ ~~ c) Soluble support 0 :).m 3) tM TRIX (MOLECULAR) ENTRAPMENT ~~~~~;;.,J~-polymer matrix 4) ENCAPSULATION membrane FIGURE I. Classification of immobilized enzymes. Covalently linked, adsorbed, and matrix-entrapped enzymes represent stage II, research on the microenvironment. Microencapsulation represents stage III, research on the intracellular environment. Further subdivision of microencapsulated enzymes will be found in Chapter 12. 4 T. M. S. CHANG matrix entrapment. In this section, detailed discussions will center on clinical analysis, urine analysis, monitoring of environmental pollution, radioimmune assay, enzyme-linked immunosorbent assay, enzyme electrodes, and other approaches involving immobilized enzymes, antibodies, and antigens. In the final section, research workers describe and discuss the perspectives of immobilized enzymes and proteins. Here, they speculate on the future potential of possible approaches, even though these may not have been extensively studied or tested at the laboratory stage. The biomedical applications of enzymes and proteins, especially in the thera peutic area, is in a very early stage of development. Much remains to be explored and studied, and the area is wide open for investigators interested in original research in a new interdisciplinary area. References Chang, T. M. S., 1972, Artificial Cells, Charles C. Thomas, Publisher, Springfield, Ill. Dunlop, R. B. (ed.), 1974, Immobilized Biochemicals and Affinity ChrOTIULtography, Plenum Press, N ew York.

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Taschenbuch. Zustand: Neu. Druck auf Anfrage Neuware - Printed after ordering - InhaltsangabeIII. Diagnostics and Public Health.- 26 The Use of Immobilized Enzymes in Automated and Semiautomated Systems of Analysis John Campbell.- 1. Enzymatic Analysis.- 2. Automation of Analytical Procedures.- 3. Immobilized Enzymes in Analysis.- 4. Conclusion.- 5. References.- 27 Immobilized Enzymes and Proteins in Urinalysis.- 1. Introduction.- 2. Carbohydrates.- 3. Nonprotein Nitrogenous Compounds.- 4. Amino Acids.- 5. Organic Acids.- 6. Proteins.- 7. Hormones.- 8. Toxicology.- 9. Miscellaneous Substances.- 10. Future Prospects.- 11. References.- 28 Immobilized Enzymes for Detection and Monitoring of Organophosphates and Carbamates.- 1. Introduction.- 2. Automatic Analysis with an Electrochemical Cell.- 3. The Continuous Aqueous Monitors, CAM-1 and CAM-2.- 4. Monitoring Air for Enzyme Inhibitors.- 5. Conclusions.- 6. References.- 29 Simplification and Automation of Radioimmunoassay by Gel Entrapment of Antibody.- 1. Introduction.- 2. Elimination of Centrifugation Step.- 3. Preparation of Gel Antibody.- 4. Elimination of Pipetting Step.- 5. Elimination of High-Molecular-Weight Interferences.- 6. Elimination of Low-Molecular-Weight Interferences.- 7. Gel Antibody RIA and Determination of High-Molecular-Weight Antigens.- 8. RIA Automation.- 9. Reuse of Solid-Phase Antibody.- 10. Summary.- 11. References.- 30 Enzyme-Linked Immunosorbent Assay.- 1. Introduction.- 2. Reagents for ELISA.- 3. Determination of Antigens.- 4. Determination of Antibodies.- 5. Conclusion.- 6. References.- 31 Application of ELISA for the Diagnosis of Bacterial Infections.- 1. Introduction.- 2. Detection and Quantitation of Antibodies.- 3. Detection and Quantitation of Antigens.- 4. Conclusions.- 5. References.- 32 Application of Enzyme-Linked Immunosorbent Assays to Parasitic Diseases.- 1. Introduction.- 2. Helminth Diseases.- 3. Protozoal Diseases.- 4. Future Developments.- 5. References.- 33 Microplate Enzyme-Linked Immunosorbent Assays in Virology.- 1. Introduction.- 2. Rubella.- 3. Cytomegalovirus.- 4. Measles.- 5. Arboviruses.- 6. Adenoviruses, Coxsackies, and Herpes.- 7. Hepatitis B.- 8. Viruses of Veterinary Importance.- 9. Plant Viruses.- 10. Conclusions.- 11. References.- 34 The Immunoadsorption of Hepatitis B Antigen from Blood Plasma.- 1. Introduction.- 2. Methodology.- 3. Immunoadsorption of Hepatitis B Antigen.- 4. Purification of Hepatitis B Antigen.- 5. References.- 35 Visual Detection of Hepatitis B Surface Antigen and Antibody.- 1. Review of the Literature.- 2. Choice of a System.- 3. Principles of the Reactions.- 4. Materials.- 5. Methods.- 6. Results.- 7. Discussion.- 8. Future Prospects.- 9. References.- 36 Enzyme Electrodes.- 1. Introduction.- 2. Enzyme Electrodes.- 3. References.- 37 Electrochemical Preparation of Enzyme-Collagen Membrane for Enzyme Electrode.- 1. Introduction.- 2. Electrochemical Preparation of Enzyme-Collagen Membrane.- 3. Properties of ADH-Collagen Membrane.- 4. Application of Enzyme-Collagen Membrane.- 5. References.- 38 Possible Biomedical Applications of the Thermal Enzyme Probe.- 1. Introduction.- 2. Thermal Enzyme Probe.- 3. Related Configurations and Devices.- 4. Simple Description of TEP.- 5. Future Directions for Biomedical Applications.- 6. References.- 39 Possible Biomedical Applications of the Volatile Enzyme Product Method.- 1. Introduction.- 2. Qualitative Description of VEP and Comparison to Gas Chromatrography-Mass Spectrometry.- 3. Theoretical Performance.- 4. Measurements of Substrates Using Volatile Products.- 5. Measurements of Nonvolatile Substrates by Use of Volatile Substrates.- 6. Measurements of Enzymes.- 7. Measurements of Inhibitors or Activators.- 8. Coupled Assays.- 9. Possible Clinical Applications.- 10. Biomedical Research Applications.- 11. Measurements on Cells.- 12. Spatial Mapping of Enzyme Distributions.- 13. References.- IV. Perspectives.- 40 Biomedical Aspects of Immobilized Enzymes and Proteins: A Physiologist's View.

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Zustand: Fine. Springfield 1972 first edition Thomas. Hardcover. Octavo, 207p., photo illustrations, cloth. Fine in VG DJ. No ownership marks.

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