Titel: Tracing Neural Connections with Horseradish ...
Verlag: John Wiley & Son Ltd, Chichester, U.K.
Auflage: First Edition.
Art des Buches: Used
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Surveys methods for tracing neural connections with the tracer horseradish peroxidase. Discusses light-microscopic tracing following extracellular injections of tracer. Describes electron microscopic applications of horseradish peroxidase histochemistry for tracing neural connections, and compares various histochemical methods. Also discusses tracing connections after intracellular injections of HRP into physiologically identified neurons. Fully treats the relevant biological principles which guide each applicationFrom the Back Cover:
With this Handbook Series, IBRO aims to fill a major gap in the world literature. The neuroscientist needs to be able to turn to whichever specialized method that is most suited to the problem he is currently studying. Methods in the Neurosciences will help to provide expert advice on exactly how to carry out the experiments, on what difficulties can occur and on the limitations of the method.
Tracing Neural Connections with Horseradish Peroxidase is the first book in the series. One of the two tracers which initiated the recent revolution in neuroanatomical methods, horseradish peroxidase offers an unusually convenient and powerful method for tracing connections of central neurons, peripheral ganglia, nerves, muscles, skin, viscera, and related structures. The entire range of applications are covered in this book, and there is a discussion of the relevant biological principles which guide these applications. For readers with no prior experience, there are extensive practical guidelines, while for the seasoned investigator there are detailed sections on background and on the potential for new directions. This handbook will be invaluable to research workers in all areas of the neurosciences, and also to anatomists, physiologists, physiological psychologists, cell biologists, and histochemists.
Contents Series Preface Preface Principles of horseradish peroxidase neurohistochemistry and their applications for tracing neural pathways?axonal transport, enzyme histochemistry and light microscopic analysis M-M. Mesulam Electron microscopic tracing of neural connections with horseradish peroxidase K. A. Carson and M-M. Mesulam Intracellular horseradish peroxidase injections for tracing neural connections G. A. Bishop and J. S. King Subject Index
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