Redox-Mediated Signal Transduction: Methods and Protocols: 1990 (Methods in Molecular Biology, 1990)

<DIV>THIS VOLUME EXPLORES THE SCOPE OF THE CELLULAR REDOX ANALYSIS AND THE IMPORTANCE OF NOT BEING LIMITED BY FREQUENTLY CHANGING AND EVOLVING TECHNOLOGY. THE CHAPTERS IN THIS BOOK COVER A WIDE RANGE OF TOPICS SUCH AS REDOX COMPONENTS IN ANIMAL AND PLANT CELLS AND THE ROLE OF REACTIVE OXYGEN SPECIES, REACTIVE NITROGEN SPECIES, AND HYDROGEN SULPHIDE IN CELL SIGNALING; MEASURING MODIFICATIONS USING FLOW CYTOMETRY, ELISA ASSAYS, AND WESTERN BLOT ANALYSIS; MEASUREMENT OF OXIDATIVE STRESS IN MITOCHONDIRA AND BIOLOGICAL SYSTEMS; AND THE USE OF THE GENETICALLY ENCODED FLUORESCENT PROBE HYPER. WRITTEN IN THE HIGHLY SUCCESSFUL <I>METHODS IN MOLECULAR BIOLOGY</I> SERIES FORMAT, CHAPTERS INCLUDE INTRODUCTIONS TO THEIR RESPECTIVE TOPICS, LISTS OF THE NECESSARY MATERIALS AND REAGENTS, STEP-BY-STEP, READILY REPRODUCIBLE LABORATORY PROTOCOLS, AND TIPS ON TROUBLESHOOTING AND AVOIDING KNOWN PITFALLS.<BR></DIV><DIV>&NBSP;</DIV><DIV>CUTTING-EDGE AND INFORMATIVE, <I>REDOX-MEDIATED SIGNAL TRANSDUCTION: METHODS AND PROTOCOLS, SECOND EDITION</I> IS A VALUABLE RESOURCE FOR BOTH NOVICE AND EXPERT RESEARCHERS WHO WANT TO EXPAND THEIR STUDIES INTO NEW AREAS AND NEW SYSTEMS IN THE EVOLVING REDOX FIELD.&NBSP;</DIV><DIV><BR></DIV>