Quarterly Journal of Experimental Physiology, Vol. 11 (Classic Reprint)

Excerpt from Quarterly Journal of Experimental Physiology, Vol. 11

My extracts were in all cases made from the ovaries. Of the cow and sheep, the corpora lutea being removed and prepared as soon as they were received from the slaughter-house, is. Within an hour or two after the animals had been killed. Occasionally the ovaries were kept overnight. In an ice-chest at a temperature of from 3° to 7° C. The method which I have used for making the extracts has been as follows - The corpora lutea. Were shelled out from the Ovaries by the aid of two pairs of forceps; the capsule or theca of each corpus luteum was then peeled Off, and the remainder Of the gland was minced and thoroughly pounded in a mortar until it formed a nearly homogeneous paste. Part of the paste was taken and weighed, mixed with a definite amount of Locke's solution, and boiled for a few minutes. It was then rapidly cooled, filtered, and made up to the original volume by the addition of distilled water. The reaction of such an extract is generally found to be neutral. This may be spoken. Of as extract of fresh corpus luteum.

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