Nitric Oxide Associated Protein (Noa) In Pea (Pisum Sativum L.): Physiological And Molecular Analyses During Bacterial Infection
Wong, Mui Yun; Huang, Jengsheng; L Davis, Eric; Wong, Mui Yun; Huang, Jengsheng; L Davis, Eric
ISBN 10: 3843372497 ISBN 13: 9783843372497
Verlag: Lap Lambert Academic Publishing, 2010
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Beschreibung
Beschreibung:
76 pages. 8.66x5.91x0.18 inches. In Stock. Bestandsnummer des Verkäufers 3843372497
Inhaltsangabe:
Nitric oxide synthase (NOS) activity was detected in pea leaf extracts using citrulline formation assay. Total protein extraction method was modified based on biochemical activities such as the use of protease inhibitors, pH and precipitation with salts and organic solvents. Physiological aspects such as the effects of various chemicals that induce systemic resistance in plants on NOS activity and immunodetection of a NOS-like protein were also studied. The NOS-like protein was partially isolated using liquid chromatography and characterized based on mammalian NOS inhibitor and co-factor requirement. Correlation of NOS activity and NOS-like gene expression during pea-bacterial interactions were investigated using Ralstonia solanacearum and Pseudomonas syringae pv pisi. NOS activity was detected using citruline formation assay. Gene expression was measured using real-time reverse transcription-polymerase chain reactions (RT-PCR) and a 348-bp probe designed from a cloned cDNA fragment of pea that was homologous to NOS of snail and AtNOS1/AtNOA1 of Arabidopsis. The possibility of NO production from various sources in the cells of pea was also discussed.
Über die Autorinnen und Autoren:
The first author is a senior lecturer at the Department of Plant Protection, and research fellow at the Institute of Tropical Agriculture, Universiti Putra Malaysia, Malaysia. She holds a PhD in Plant Pathology and Biotechnology (North Carolina State University, Raleigh, USA). The co-authors are attached with North Carolina State University.
The first author is a senior lecturer at the Department of Plant Protection, and research fellow at the Institute of Tropical Agriculture, Universiti Putra Malaysia, Malaysia. She holds a PhD in Plant Pathology and Biotechnology (North Carolina State University, Raleigh, USA). The co-authors are attached with North Carolina State University.
The first author is a senior lecturer at the Department of Plant Protection, and research fellow at the Institute of Tropical Agriculture, Universiti Putra Malaysia, Malaysia. She holds a PhD in Plant Pathology and Biotechnology (North Carolina State University, Raleigh, USA). The co-authors are attached with North Carolina State University.
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Bibliografische Details
Titel: Nitric Oxide Associated Protein (Noa) In Pea...
Verlag: Lap Lambert Academic Publishing
Erscheinungsdatum: 2010
Einband: Paperback
Zustand: Brand New