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Monoclonal Antibodies: A Practical Approach

Phil S. Shepherd, Chris Dean

Verlag: Oxford Univ Pr, 1999
ISBN 10: 0199637237 / ISBN 13: 9780199637232
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Titel: Monoclonal Antibodies: A Practical Approach

Verlag: Oxford Univ Pr

Erscheinungsdatum: 1999

Einband: Hardcover

Zustand: Fine

Auflage: First Edition.


with no gift inscription or remarks inside. 479 pages with the index. hardcover.Large Heavy Books May Need More Payment For Overseas Delivery. Buchnummer des Verkäufers 21827

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Inhaltsangabe: Monoclonal Antibodies: A Practical Approach covers the preparation, testing, derivation, and applications of monoclonal antibodies. New immunological techniques incorporating tried and tested methodologies are described, making the book of interest to established and inexperienced immunologists. Both the standard somatic hybridization technique and recombinant techniques, including the use of phage libraries, for the preparation of rodent and human monoclonal antibodies are described. Protocols for both the small and large scale production are detailed, as well as purification and labelling (with both radioisotopes and non-radioisotopes) methods. The applications of monoclonal antibodies in immunoblotting, enzyme linked immunoassays, immunofluorescence, and FACS analysis are all covered in detail. Finally protocols are given for the use of monoclonal antibodies in rheumatoid arthritis, tissue typing, detecting DNA modified during chemotherapy, and in the clinical analysis of transplantation samples for malignancy. This book will therefore be an invaluable laboratory companion to anyone using monoclonal antibodies in their research.

Über den Autor: Phil S. Shepherd, Department of Immunology, The Guy's, King's College, and St Thomas's Hospitals, London. Chris Dean, Institute for Cancer Research, Sutton, Surrey.

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