Preface...
Table of Contents...
Contributing Authors...
Part I Fundamentals
1. Metabolic Profiling: Topic, Challenges, Perspective
Helen G. Gika, Georgios A. Theodoridis, and Ian D. Wilson
2. Quality Control and Validation Issues in LC-MS Metabolomics
Olga Begou, Helen G. Gika, Georgios A. Theodoridis, and Ian D. Wilson
3. Data Treatment for LC-MS Untargeted Analysis
Samantha Riccadonna and Pietro Franceschi
4. Bio- and Chemoinformatics Approaches for Metabolomics Data Analysis
Michael Witting
Part II Methods
5. HILIC- MS/MS Multi-Targeted Method for Metabolomics Applications
Christina Virgiliou, Helen G. Gika, and Georgios A. Theodoridis
6. Ion Pair Chromatography for Endogenous Metabolites LC-MS Analysis in Tissue Samples Following Targeted Acquisition
Filippos Michopoulos
7. LC-MS Untargeted Analysis
Elizabeth Want
8. NMR Based Metabolic Profiling Procedures for Biofluids, and Cell and Tissue Extracts
Dimitra Benaki and Emmanuel Mikros
9. Untargeted GC-MS Metabolomics
Μatthaios-Εmmanouil P. Papadimitropoulos, Catherine G. Vasilopoulou, Christoniki Maga-Nteve, and Maria I. Klapa
10. Rat Fecal Metabolomics Based Analysis
Olga Deda, Helen G. Gika, and Georgios A. Theodoridis
11. GC-MS Metabolomic Profiling of Protic Metabolites Following Heptafluorobutyl Chloroformate Mediated Dispersive Liquid Microextraction Sample Preparation Protocol
Petr Hušek, Zdeněk Švagera, Dagmar Hanzlíková, Iva Opekarová, Lucie Řimnáčová, Helena Zahradníčková, and Petr Šimek
12. Sheathless Capillary Electrophoresis-Mass Spectrometry for the Profiling of Charged Metabolites in Biological Samples
Rawi Ramautar
Part III Plant/Food and Other Applications
13. Two-Phase Extraction for Comprehensive Analysis of the Plant Metabolome by NMR
Jan Schripsema and Denise Dagnino
14. NMR Spectroscopy Protocols for Food Metabolomics Applications
Evangelia Ralli, Maria Amargianitaki, Efi Manolopoulou, Maria Misiak, Georgios Markakis, Sofia Tachtalidou, Alexandra Kolesnikova, Photis Dais, and Apostolos Spyros
15. Direct Injection Analysis of Fruit VOCs by PTR-ToF-MS: The Apple Case Study
Brian Farneti
16. LC-MS Untargeted Protocol for the Analysis of Wine
Panagiotis Arapitsas and Fulvio Mattivi
Part IV Life Science Applications
17. Tissue Multiplatform-Based Metabolomics/Metabonomics for Enhanced Metabolome Coverage
Panagiotis A. Vorkas, M.R. Abellona U, and Jia V. Li
18. UHPLC-HRMS Analysis for Steroid Profiling in Serum (Steroidomics)
Federico Ponzetto, Julien Boccard, Raul Nicoli, Tiia Kuuranne, Martial Saugy, and Serge Rudaz
19. Metabolomics in Human Acute-Exercise Trials: Study Design and Preparation
Aikaterina Siopi and Vassilis Mougios
This volume explores the different approaches and techniques used by researchers to study the recent challenges and developments in metabolic profiling. This book is divided into IV parts. Part I contains chapters that highlight basic concepts, such as experimental design, data treatment, metabolite identification, and harmonization. Part II describes experimental protocols for both targeted and untargeted metabolomics covering the basic analytical technologies: LC-MS, GC-MS, NMR and CE-MS. In addition the protocols describe methods for the study of tissues, feces, blood and other types of biological samples as well as the application of chemical derivatization for GC-MS. Parts III and IV present the use of metabolomics in the study of food, plants and the life sciences, with examples from the quest for the discovery of disease biomarkers, physical exercise omics and metabolic profiling of food, fruit and wine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and thorough, Metabolic Profiling: Methods and Protocols is a valuable resource for researchers who are interested in expanding their knowledge of this rapidly developing field.
