Druck auf Anfrage Neuware - Printed after ordering - Advances in the field of cell biology have always been closely related to the development of quantitative analytical methods that can be applied to individual cells or cell organelles. Almost from the early stages following the invention of the microscope, the investigator has been keenly interested in obtaining informa tion on the functionality of single cells and how cells perform under different sets of experimental conditions. Although cells could be viewed in the microscope for a few hundred years, only since the relatively recent application of autoradiography did we come to realize that, although cells may visually appear very much alike, they are quite different in their functional capacity. The quest to understand these differences in a cell population lead to a new series of techniques for labeling and quantitating DNA content and similar approaches have driven the develop ment of methods for analyzing various other cellular properties. The development of new analytical techniques follows the age old pattern of applying successes of the past with current inno vation, logic and new biological information. Results from auto radiography expanded the concept of the cell cycle from inter phase and mitosis to the more definitive GO/GI, Sand G2/M phases. This new knowledge lead to the development of techno logy to measure and analyze various parameters related to the cell cycle.

Bestandsnummer des Verk�ufers 9783642629785

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Bibliografische Details

Titel: In Living Color : Protocols in Flow Cytometry and Cell Sorting
Verlag: Springer Berlin Heidelberg
Ver�ffentlichungsdatum: 2013
Sprache: Englisch
ISBN-10: 3642629784
ISBN-13: 9783642629785
Bindung: Taschenbuch
Zustand: Neu

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Inhaltsangabe

Advances in the field of cell biology have always been closely related to the development of quantitative analytical methods that can be applied to individual cells or cell organelles. Almost from the early stages following the invention of the microscope, the investigator has been keenly interested in obtaining informa� tion on the functionality of single cells and how cells perform under different sets of experimental conditions. Although cells could be viewed in the microscope for a few hundred years, only since the relatively recent application of autoradiography did we come to realize that, although cells may visually appear very much alike, they are quite different in their functional capacity. The quest to understand these differences in a cell population lead to a new series of techniques for labeling and quantitating DNA content and similar approaches have driven the develop� ment of methods for analyzing various other cellular properties. The development of new analytical techniques follows the age old pattern of applying successes of the past with current inno� vation, logic and new biological information. Results from auto� radiography expanded the concept of the cell cycle from inter� phase and mitosis to the more definitive GO/GI, Sand G2/M phases. This new knowledge lead to the development of techno� logy to measure and analyze various parameters related to the cell cycle.

Rese�a del editor

A contribution towards making this increasingly valuable technology accessible to researchers, including the students, post-doctoral scholars, and technicians gathering the knowledge inherent in this integration between analysis and physical isolation/purification methodologies. A step-by-step approach to the methodology for measuring various attributes demonstrated in the particular cells of interest is provided, as is a myriad of resources to fuel the curiosity and answer questions of both new and adept users. This book stems from the editors'experiences managing flow cytometry/cell sorting core facilities for the emerging researchers, in particular in developmental, cellular, and molecular biology.

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