Laboratory Manual of Microbiology

Vivek Kumar

Verlag: Scientific Publishers, 2011
ISBN 10: 817233706X / ISBN 13: 9788172337063
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This laboratory manual of microbiology has been written to meet the needs of students taking microbiology as major or subsidiary subject. The intention is to provide the students with well organized, user-friendly tool to better enable them to understand laboratory aspects of microbiology as well as to hopefully make learning laboratory material and preparing for independent player of a given experiment. Each exercise provides step-by-step procedure to complete the assignment successfully and easily. The lab exercises are designed to give the student "hands-on" laboratory experience to better reinforce certain topics discussed in exercise. The glossary is included covering terms as well as basic, discipline-specific terminology from microbiology that will be helpful to its readers. The main contents of the manual are: Microbiology laboratory practices and safety rules, Basic laboratory techniques, Microscopy, Staining and motility techniques, Environmental microbiology, Microbiological culture techniques, Growth of lactose fermenting and non fermenting microbes, Medical microbiology, Environmental effect on bacterial growth, Application of microbiology, Microbiology of milk and Appendices. The academic level of the book is graduate, post graduate students, research workers, teachers and scientists dealing with basic and applied aspects of microbiology. Contents: Exercises 1 Microbiology laboratory practices and safety rules I BASIC LABORATORY TECHNIQUES 2. Media preparation 2.1. Sub culturing or picking off: 2.2. Broth cultures 3. Sterilization and disinfection 4. Inoculation and other aseptic procedures 4.1. Using a wire loop 4.2. Flaming procedure 4.3. Using a pipette 4.4. Flaming the neck of bottles and test tubes 5. Inoculation using a pipette 6. Pouring the media in Petri plate 7. Use of spreader 8. Spread plate technique II MICROSCOPY 9. Use and care of microscope 10. Dark field microscopy 10.1. Bright field microscopy 10.2. Resolving power of microscope 10.3. Phase contrast field microscopy 10.4. Fluorescence microscopy 10.5. Electron microscopy 11. Ocular micrometery III STAINING AND MOTILITY TECHNIQUES 12. Staining preparations 13. Smear making 14. Simple staining 15. Gram staining of bacteria 16. Capsule staining of bacteria 17. Capsule staining by negative, indirect or background staining 18. Acid fast (Ziehl-Neelsen) staining of bacteria 19. Endospore staining of bacteria 20. Dorner?s method of endospore staining 21. Bacterial flagella staining 22. Viability staining technique of bacteria 23. Lactophenol cotton blue staining of fungi 24. Staining of Vesicular Arbuscular Mycorrhiza (VAM) 25. Staining of actinomycetes culture 26. Hanging drop slide for bacterial motility 27. Motility Determination (Tube method) 28. Slide technique for protozoa motility IV BIOCHEMICAL ACTIVITIES OF MICROBES 29. Urease activity of bacteria 30. Nitrate reduction test 31. Catalase test 32. Lipid hydrolysis test 33. Oxidase test 34. DNase production test 35. Starch hydrolysis test or amylase production test 36. Gelatin hydrolysis or production of gelatinase 37. IMVIC tests 37.1. Indole Test 37.2. Methyl Red and Voges-Proskauer Tests 37.3. Citrate Test 38. Hydrogen sulfide production and motility 39. TSI (Triple Sugar Iron) and KIA (Kligler`s Iron Agar) 40. Casein hydrolysis 41. Estimation of phytohormone IAA production from bacteria 42. Estimation of ammonia production by bacteria 43. Solubilization of mineral phosphate by microbes V ENVIRONMENTAL MICROBIOLOGY 44. Multiple tube fermentation tests 44.1. Presumptive coliform test 44.2. Confirmed coliform test 44.3. Completed coliform test 45. Counting of Ascaris eggs in raw or treated sewage water 46. Determination of dissolved oxygen (DO) of water 47. Determination of biological oxygen demand (BOD) of water 48. Determination of chemical oxygen Printed Pages: 214. Buchnummer des Verkäufers 65462

Bibliografische Details

Titel: Laboratory Manual of Microbiology
Verlag: Scientific Publishers
Erscheinungsdatum: 2011
Einband: Hardcover
Zustand: New

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