Dedication...
Preface...
Table of Contents...
Contributing Authors...
Part I GAG Structures
1. One-Pot Enzymatic Synthesis of Heparin from N-Sulfoheparosan
Li Fu and Robert J. Linhardt
2. Solution NMR Spectroscopy for Characterizing Protein-Glycosaminoglycan Interactions
Prem Raj B. Joseph, Krishna Mohan Sepuru, Krishna Mohan Poluri, and Krishna Rajarathnam
3. Metabolic Labeling of Proteoglycans and Analysis of Their Synthesis and Sorting in Filter-Grown and Polarized Epithelial Cells
Kristian Prydz and Ravi Adusumalli
4. Fluorous-Tag Assisted Synthesis of GAG-Like Oligosaccharides
José L. de Paz and Pedro M. Nieto
5. Aqueous Molecular Dynamics for Understanding Glycosaminoglycan Recognition by Proteins
Balaji Nagarajan and Umesh R. Desai
6. A Non-Radioactive Method to Measure Hyaluronan Synthase Activity
Davide Vigetti, Evgenia Karousou, Manuela Viola, and Alberto Passi
7. A Glycoproteomic Approach to Identify Novel Proteoglycans
Fredrik Noborn, Mahnaz Nikpour, Andrea Persson, Carina Sihlbom, Jonas Nilsson, and Göran Larson
8. Analysis of Protein-Glycosaminoglycan Interactions using Traveling Wave Ion Mobility Mass Spectrometry
Robert V. Williams and I. Jonathan Amster
9. Ascidian (Chordata-Tunicata) Glycosaminoglycans: Extraction, Purification, Biochemical, and Spectroscopic Analysis
Felipe C. O. B. Teixeira and Mauro S. G. Pavão
10. Quantitative Disaccharide Profiling of Glycosaminoglycans from Two Different Preparations by PMP and Deuterated PMP Labeling
Meng Zhang, Yi An, and Lijuan Zhang
11. Preparation and Characterization of Heparan Sulfate Derived Oligosaccharides to Investigate Protein-GAG Interaction and HS Biosynthesis Enzyme Activity
Cédric Laguri, Rabia Sadir, Evelyne Gout, Romain R. Vivès, and Hugues Lortat-Jacob
12. Heparan Sulfate Structure: Methods to Study N-sulfation and NDST Action
Anders Dagälv, Anders Lundequist, Beata Filipek-Górniok, Tabea Dierker, Inger Eriksson, and Lena Kjellén
13. Methods for the Production of Recombinant Heparosan: A Critical Heparin Precursor, from Non-Pathogenic E.coli Strains
Anindita Roy, Varun Garg, Sai K Mehta, Alessandro Rossi, and Balagurunathan Kuberan
14. De novo Sequencing of Heparin/Heparan Sulfate Oligosaccharides by Chemical Derivatization and LC-MS/MS
Quntao Liang and Joshua S. Sharp
15. Production and HPLC-Based Disaccharide Analysis of Xyloside-Primed Glycosaminoglycans
Andrea Persson, Emil Tykesson, Ulf Ellervik, and Katrin Mani
16. Nuclear Magnetic Resonance Methods in Structural Characterization of Glycosaminoglycans
Vitor H. Pomin and Barbara Mulloy
17. Aggrecan: Approaches to Study Biophysical and Biomechanical Properties
Hadi Tavakoli Nia, Christine Ortiz, and Alan Grodzinsky
18. Chromatographic Molecular Weight Measurements for Heparin: Its Fragments and Fractions, and Other Glycosaminoglycans
Barbara Mulloy and John Hogwood
19. Mass Spectrometric Methods for the Analysis of Heparin and Heparan Sulfate
Miroslaw Lech, Ishan Capila, and Ganesh V. Kaundinya
20. Affinity Purification of Glycosylphosphatidylinositol-Anchored Proteins by Alpha-Toxin
Kevin Huang and Sungjin Park
21. Thermodynamic Affinity and Nature of Forces Defining Glycosaminoglycan: Protein Systems Using Fluorescence Spectroscopy
Rio S. Boothello and Umesh R. Desai
22. Isolation and Characterization of Heparan Sulfate Containing Amyloid Precursor Protein Degradation Products
Katrin Mani
23. Chemical Approaches to Prepare Modified Heparin and Heparosan Polymers for Biological Studies
Karthik Raman and Sailaja Arungundram
24. Glycosaminoglycan Quality Control by Monosaccharide Analysis
Yiran Zhang, Xuexiao Ma, and Lijuan Zhang
25. Characterizing Thermodynamics of Protein-Glycosaminoglycan Interactions using
This second edition volume expands on the previous edition with new information on the structural analysis of glycosaminoglycans (GAGs); chemical and enzymatic synthesis of GAGs; biophysical, biochemical, and computational analysis of GAG-protein interactions; molecular and genetic approaches to manipulating GAG cell biology; and molecular and genetic methods involving animal models and organoids. This volume also offers insight and guidance to non-glycoscience researchers who are performing advanced experiments on GAGs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Glycosaminoglycans: Methods and Protocols, Second Edition is a valuable experimental manual for practicing researchers including graduate and post-doctoral fellows, and provides them with the latest methodologies and practical tips to overcoming barriers in understanding the chemistry and biology of GAGs.
