Gene Cloning and Manipulation

Christopher Howe

Verlag: Cambridge University Press, 2007
ISBN 10: 0521817935 / ISBN 13: 9780521817936
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Now fully updated to reflect recent advances, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams. • Covers a broad range of host organisms, from E. coli to mammals • Focuses on the underlying principles thereby equipping the reader to devise appropriate strategies for novel situations and for use in their own research • Contains example scenarios, which allow the reader to test their understanding of the material and apply it to ‘real-life’ research situations Contents Preface; 1. The tools for the job; 2. Polymerase chain reaction; 3. Simple cloning; 4. Other vector systems for E. Coli; 5. Making libraries; 6. Screening libraries; 7. Modification and mutagenesis; 8. Use of cloned DNA; 9. Using other organisms; 10. Examples. Printed Pages: 276 with 131 line diagrams, 9 tables and 131 figures. Size: 190 x 250 Mm. Buchnummer des Verkäufers 017672

Über diesen Titel:

Inhaltsangabe: Now fully updated to reflect recent advances, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams.

Book Description: Fully updated to reflect recent advances, this introduction provides a broad but concise coverage of recombinant DNA techniques. It is written specifically for students and researchers who want to use this technology, with emphasis placed on understanding the underlying concepts, thereby enabling the reader to devise their own strategies.

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Bibliografische Details

Titel: Gene Cloning and Manipulation
Verlag: Cambridge University Press
Erscheinungsdatum: 2007
Einband: Hardcover
Zustand: New
Zustand des Schutzumschlags: New
Auflage: Second Edition.

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Howe, Christopher
Verlag: Cambridge university press 1995 (1995)
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Buchbeschreibung Cambridge university press 1995, 1995. Kartoniert, Format groß 8°, 210 Seiten, ein regulär ausgesondertes Bibliotheksexemplar aus einer wissenschaftlichen Bibliothek, keine Markierungen / Anstreichungen im Text, das Buch befindet sich in einem sehr guten Zustand --- hardcover, 210 pages, Lib.Ex., no marks / underlines, the book is in a very good condition. Bezahlung mit PAYDIREKT möglich. Artikel-Nr. 11340

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