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Electron Microscopy in Biology: A Practical Approach (Practical Approach Series)

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ISBN 10: 0199632154 / ISBN 13: 9780199632152
Verlag: IRL Press
Gebraucht Zustand: Fine Softcover
Verkäufer Robinson Street Books, IOBA (Binghamton, NY, USA)

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Titel: Electron Microscopy in Biology: A Practical ...

Verlag: IRL Press



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This volume provides detailed protocols for a wide range of procedures relating to the electron microscopy of tissues, cells, and their components with the emphasis on biomedical applications. The fundamental methods for specimen preparation, tissue fixation, embedding, and sectioning are covered in depth and are followed by techniques in freeze-substitution, immunogold labelling, auto- radiography, and enzyme histochemistry. Although the technical details in the book relate mainly to tissues and cells, many of the procedures can be adapted for isolated organelles and membrane fractions, viral particles, macromolecular assemblies, and large proteins. This is a broadly based compilation of the most important specimen preparation techniques and their applications. It will be of interest to researchers and advanced students working in almost every biomedical discipline, plus technicians in electron microscopy units.


'The book successfully achieves its goal of presenting a wide variety of electron-microscopic methods ... the chapters are succinct, easy to follow, authoritative, and nicely illustrated ... the contributors should be praised for capturing the broad usefulness of electron microscopy in contemporary biomedical research and pathology. The book convincingly demonstrates that electron microscopy continues to provide novel morphological information because of the ongoing development of imaginative new methods.' Donald M. McDonald, University of California, Bookshelf, April 1993 'EM histologists will find this a very useful book' Comp. Biochem. Physiol. Vol. 103A, No. 3, 1992 'I am a great fan of the Practical Approach series and am almost becoming a collector of these extremely useful and reasonably priced laboratory aids. Robin Harris has gathered together an impressive set of contributors to produce a book that will be of great use to anyone interested in the practical aspects of biological electron microscopy ... an excellent book on transmission EM techniques which I would recommend both for personal libraries and as a general aid in the laboratory.' Chris Hawes, Journal of Microscopy 'When reading through, one gets the clear impression backed up by personal experience, that the authors themselves really use the methods and know what they are talking about ... this book will play a very useful role.' Cell Biology International Reports, Vol.16, No.2, 1992

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