1. A Method of Bone-Metastatic Tumor Progression Assessment in Mice Using Longitudinal Radiography
Matthew R. Eber, Juan Miguel Jiménez-Andrade, Christopher M. Peters, and Yusuke Shiozawa
2. Optical Imaging of Matrix Metalloproteinases Activity in Prostate Tumors in Mice
Susy Kim and Gagan Deep
3. Method to Development of PET Radiopharmaceutical for Cancer Imaging
Naresh Damuka and Kiran Kumar Solingapuram Sai
4. PET Use in Cancer Diagnosis, Treatment, and Prognosis
Naresh Damuka, Meghana Dodda, Kiran Kumar Solingapuram Sai
5. Immunofluorescence-Based Method to Assess Cancer Biomarker in the Hypoxic Region of the Tumor
Leslimar Rios-Colon, Susy Kim, Yixin Su, Deepak Kumar, and Gagan Deep
6. Zebrafish Xenograft Model to Study Human Cancer
Ranganatha R. Somasagara and TinChung Leung
7. Assessing Oligomerization Status of Mitochondrial OXPHOS Complexes via Blue Native Page
Jordan Woytash, Joseph A Inigo, and Dhyan Chandra
8. In Vitro Cell Impedance Assay to Examine Antigen-Specific T-Cell-Mediated Melanoma Cell Killing to Support Cancer Immunotherapy Drug Discovery
Elizabeth R. Stirling and David R. Soto-Pantoja
9. Methods to Detect Nitric Oxide and Reactive Nitrogen Species in Biological Sample
Sharanjot Kaur, Kunj Bihari Gupta, Sandeep Kumar, Shishir Upadhyay, Anil Kumar Mantha, and Monisha Dhiman
10. Study of Rotary Cell Culture System Induced Microgravity Effects on Cancer Biomarkers
Ragini Singh and Rana P. Singh
11. A Calcium Imaging Approach to Measure Functional Sensitivity of Neurons
Joshua J. Wheeler, John M. Davis, and Santosh K. Mishra
12. Zymography and Reverse Zymography for Testing Proteases and Their Inhibitors
Preety Choudhary, Vineet Kumar Mishra, and Snehasikta Swarnakar
13. Method of Preparation of Cigarette Smoke Extract to Assess Lung Cancer-Associated Changes in Airway Epithelial Cells
Hina Agraval, Jiten R. Sharma, and Umesh C.S. Yadav
14. Air-Liquid Interface Culture Model to Study Lung Cancer-Associated Cellular and Molecular Changes
Hina Agraval, Jiten R. Sharma, Neeraj Dholia, and Umesh C.S. Yadav
15. Co-Immunoprecipitation-Blotting: Analysis of Protein-Protein Interactions
Li Tan and Raghunatha R. Yammani
16. Methods to Assess Oxidative DNA Base Damage Repair of Apurinic/Apyrimidinic (AP) Sites Using Radioactive and Non-Radioactive Oligonucleotide-Based Assays
Kunj Bihari
This detailed volume explores numerous methods used in basic science laboratories to characterize cancer-related biomarkers, vital for better managing cancer burden, including cancer risk assessment, cancer diagnosis, determining cancer progression, and therapeutic response. From a radiography method to an examination of single-cell RNA-seq and computational analysis tools in cancer research, this book delves into many techniques that could provide valuable molecular information about the tumor and its microenvironment components. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Cancer Biomarkers: Methods and Protocols offers researchers multiple helpful ways to study cancer-associated molecular biomarkers.
