Biotechnology (A Multi-Volume Comprehensive Treatise): Recombinant Proteins, Monoclonal Antibodies and Therapeutic Genes (Volume 5a)

Biotechnology Second, Completely Revised Edition Edited by H.-J. Rehm and G. Reed in cooperation with A. Pühler and P. Stadler This fully revised and expanded Second Edition takes into account all recent developments in biotechnology. It is extraordinarily broad in scope, up-to-date, carefully structured and well-balanced. "Biotechnology" considers both basic concepts and widely-differing industrial applications: it is a successful synthesis of theory and practice. Any scientist aiming for success in industrial microbiology, biochemistry, molecular biology and chemical engineering must turn to "Biotechnology". Volume 5a: Proteins are the molecules of choice when highly specific medical treatments or other highly specific technical properties are requried. The first chapters provide an overview of protein structure and function and of the design and uses of recombinant proteins. Options and issues of antibody therapies are discussed in chapters ranging from the design and manufacture of antibodies through pre-clinical testing and clinical development. The new approach to treatment of disease by targeted gene therapy is reviewed demonstrating the extent of challenges still to be met before gene therapy products can be used successfully. The rapidly changing global regulatory environment and pharmacoeconomic issues for products of biotechnology are also highlighted. Topics included are: Structure and Function of Proteins • Recombinant Proteins: In vitro Folding • Medical Applications • Industrial Applications • Monoclonal Antibodies: Engineering and Expression • Manufacture • Preclinical Testing • Therapeutic Applications • Case Studies • Gene Therapy: Vectors • Large-Scale Plasmid DNA Manufacturing • Regulatory and Economic Aspects