Antibody Phage Display: Methods and Protocols (Methods in Molecular Biology). Dieser Artikel ist nicht verfügbar.Antibody Phage Display: Methods and Protocols (Methods in Molecular Biology)

The closing years of the 19th century and the start of the 20th century witnessed the emergence of microbiology and immunology as discrete sci- tific disciplines, and in the work of Roux and Yersin, perhaps the first benefits of their synergy―immunotherapy against bacterial infection. As we advance into the new millennium, microbiology and immunology again offer a c- ceptual leap forward as antibody phage display gains increasing acceptance as the definitive technology for monoclonal production and unleashes new - portunities in immunotherapy, drug discovery, and functional genomics. In assembling Antibody Phage Display: Methods and Protocols, we have aimed to produce a resource of real value for scientists who have followed the development of phage display technology over the past decade. The founding principles of phage display have always held an elegant simplicity. We hope that readers will find similar clarity in the technical guidance offered by the book’s contributors. In meeting our objectives, we have tried to cover the broad scope of the technology and the key areas of library construction, scre- ing, antibody modification, and expression. Of course, the technology cont- ues to advance apace, but we trust that readers will be able to gage the potential of phage display from our coverage, that some of its subtleties will emerge, and that our selection of methods will prove appealing. We are indebted to all the contributing authors for sharing their expertise with the wider scientific community.

Antibody phage display, the definitive technology for monoclonal production, has now advanced to a stage where it can be performed in nonspecialized research laboratories. In Antibody Phage Display: Methods and Protocols, Philippa M. O'Brien and Robert Aitken combine in one volume a comprehensive collection of established antibody phage display protocols, each accompanied by authoritative guidance that will enable the nonspecialist to carry them out successfully. Coverage spans the construction of antibody libraries, the selection of antibody clones with the desired properties, and their modification, expression, and purification. Each readily reproducible method is described by a hands-on expert in step-by-step detail and includes a wealth of practical advice not found in the scientific literature. Extensive notes discuss pitfalls to avoid and offer many possible alternative methods to suit special research situations. An overview by one of the world's leading experts in antibody phage display, Dr. Hennie R. Hoogenboom, surveys the current status of the field and the future of the technology.
Comprehensive and highly practical, Antibody Phage Display: Methods and Protocols provides biochemists, molecular biologists, and immunologists with a gold-standard reference guide to the successful isolation, modification, and expression of recombinant antibodies using today's powerful phage display technology.