Techniques In Molecular Biology. Hardbound Library Edition [Hardcover] [Jul 06, 2000] Agrawal, Suraksha [Hardcover] [Jan 01, 2017] Agrawal, Suraksha - Hardcover

Techniques in Molecular Biology

Hardcover. Zustand: As New. Contents 1. Nucleic acid extraction. 2. Polymerase chain reaction. 3. Electrophoresis techniques. 4. Reverse transcriptase PCR (Gene expression analysis). 5. Real time PCR. 6. Short Tandem Repeat (STR) genotyping. 7. Alu insertion genotyping. 8. Restriction Fragment Length Polymorphism (RFLP). 9. Amplification mutation detection system (ARMS). 10. Single Stranded Conformation Polymorphism (SSCP). 11. Nucleic acid blotting techniques. 12. Role of microarray techniques in present day molecular biology. 13. DNA sequencing. 14. Multiplex PCR and automated DNA fragment analysis by Gene scanning. 15. DNA recombinant technology. 16. Most important buffers and media used in molecular biology laboratory. Glossary. Index. Completion of the human genome project is an important mile stone in the understanding of the complex processes involved in modern biology. It is a step towards understanding more precisely the function of each gene which may in turn lead in future to practice molecular and individualized medicine. Practice of molecular medicine is a two fold interaction of intensive basic and applied research. All the techniques which are being used in molecular biology are highly sophisticated and advanced. Moreover these techniques are unique for a set of hypothesis being proposed to answer a specific question. Various molecular techniques have application not only in the field of biotechnology forensic science and pharmaceutical research but also have a wide potential in medical sciences. In this manual we have made an attempt to provide the basic back ground of each technique followed by detailed recipe and then how to carryout different experiments. A separate chapter on a brief overview of all the statistical methods which can be used to analyze the biological data has been included. 330 pp. Artikel-Nr. 70429

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