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Molecular Histochemical Techniques (Springer Lab Manuals)

 
9784431702313: Molecular Histochemical Techniques (Springer Lab Manuals)
ISBN 10: 4431702318 ISBN 13: 9784431702313
Verlag: Springer, 2000

Inhaltsangabe

Detailed protocols for analyzing DNA strand breaks, specific RNA/DNA expressions, and DNA binding proteins such as transcription factors at the individual cell level. It describes in situ nick translation and TUNEL in detail, along with radioactive and non-radioactive methods for in situ hybridization. Of special significance is the description of Southwestern histochemistry, which makes it possible to analyze the expression of transcription regulatory factors in individual cells. This volume provides an overview of the current status of molecular histochemistry along with practical how-to-do-it details. The methodology is set out in easy-to-follow language by leading scientists working in the field today. Both a rich source of the latest information and a practical lab manual.

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Molecular Histochemical Techniques presents detailed protocols for analyzing DNA strand breaks, specific RNA/DNA expressions, and DNA binding proteins such as transcription factors at the individual cell level. In situ nick translation and TUNEL are described in detail, along with radioactive and nonradioactive methods for in situ hybridization. Of special significance is the description of Southwestern histochemistry, a new development in molecular histochemistry, which makes it possible to analyze the expression of transcription regulatory factors in individual cells. This volume provides an overview of the current status of molecular histochemistry along with practical how-to-do-it details. Methodology is set forth step-by-step in easy-to-follow language by leading scientists who are working in the field today. For researchers, laboratory technicians, and graduate students, the book serves as both a rich source of the latest information and a practical lab manual.

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Verlag
Springer
Erscheinungsdatum
2000
Sprache
Englisch
ISBN 10 
4431702318
ISBN 13 
9784431702313
Einband
Spiralbindung
Anzahl der Seiten
276
Herausgeber
Koji Takehiko
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