ELISA - Enzyme Linked Immunosorbent Assay: reliable and cheap method for the detection of food allergens - Softcover

Iqbal, Amjad

 
9783848436637: ELISA - Enzyme Linked Immunosorbent Assay: reliable and cheap method for the detection of food allergens
Softcover
ISBN 10: 3848436639 ISBN 13: 9783848436637
Verlag: LAP LAMBERT Academic Publishing, 2012

Inhaltsangabe

In ELISA formats there are several parameters that are common and critical to assay performance and should be considered during the optimization process, including antibodies concentration, antigen concentration, incubation time of blocking, substrate solution and washing solution. Though immunoassays offer a specific, sensitive and rapid method to detect and quantify even trace amounts of allergens in foodstuffs, certain processes can denature, alter or destroy proteins so that they are no longer detectible by assay using antibodies. When using monoclonal antibodies, there is a great chance of obtaining false negatives in the detection. Polyclonal antibodies are recommended for ELISA techniques as the risk of obtaining false negatives is reduced as compared to monoclonal antibodies which are quite specific.

Die Inhaltsangabe kann sich auf eine andere Ausgabe dieses Titels beziehen.

Verlag
LAP LAMBERT Academic Publishing
Erscheinungsdatum
2012
Sprache
Englisch
ISBN 10 
3848436639
ISBN 13 
9783848436637
Einband
Taschenbuch
Anzahl der Seiten
80
Kontakt zum Hersteller
Nicht verfügbar
Verantwortliche Person
Editions Vie
customerservice@vdm-vsg.de

Brivibas gatve 197
Riga
1039
Lettland