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RNA Interference in insect cells infected with baculovirus: A universal transgene silencing approach - Softcover
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Baculovirus?insect cell system (BICS) is considered one of the most efficient eukaryotic gene expression systems. Establishing a universal gene silencing (UGS) system is very important due to the increasing number of studies using RNA interference (RNAi) with BICS. In this work, the enhanced green fluorescent protein (EGFP) was used as the RNAi consistent target sequence located downstream of a depressant insect-neurotoxin gene, LqqIT2 used as a model of the gene of interest. Small interfering RNA (siRNA) and inverted repeats of EGFP gene (IR-EG) were examined in targeting the EGFP-LqqIT2 (EL)-fusion mRNA or LqqIT2-EGFP (LE)-bicistronic mRNA for degradation. Suppression efficiencies using these inducers were examined transiently and stably in uninfected and infected insect Sf9 cells.
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Über die Autorin bzw. den Autor
Tamer Salem is a Research Associate Professor in Molecular Virology. He is working in AGERI, Egypt. He obtained his Ph.D. from Microbiology Department in University of Florida, USA. He had varies post- doctoral and research associate positions in Miami University (Ohio), University of Florida (Florida), and Michigan State University (East Lansing).
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RNA Interference in insect cells infected with baculovirus | A universal transgene silencing approach
Verlag:
LAP LAMBERT Academic Publishing, 2010
ISBN 10: 384335135X
ISBN 13: 9783843351355
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Taschenbuch. Zustand: Neu. RNA Interference in insect cells infected with baculovirus | A universal transgene silencing approach | Tamer Salem | Taschenbuch | 144 S. | Englisch | 2010 | LAP LAMBERT Academic Publishing | EAN 9783843351355 | Verantwortliche Person für die EU: BoD - Books on Demand, In de Tarpen 42, 22848 Norderstedt, info[at]bod[dot]de | Anbieter: preigu. Artikel-Nr. 107296026
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RNA Interference in insect cells infected with baculovirus : A universal transgene silencing approach
Verlag:
LAP LAMBERT Academic Publishing, 2010
ISBN 10: 384335135X
ISBN 13: 9783843351355
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Zustand: Sehr gut. Zustand: Sehr gut | Sprache: Englisch | Produktart: Bücher | Baculovirus¿insect cell system (BICS) is considered one of the most efficient eukaryotic gene expression systems. Establishing a universal gene silencing (UGS) system is very important due to the increasing number of studies using RNA interference (RNAi) with BICS. In this work, the enhanced green fluorescent protein (EGFP) was used as the RNAi consistent target sequence located downstream of a depressant insect-neurotoxin gene, LqqIT2 used as a model of the gene of interest. Small interfering RNA (siRNA) and inverted repeats of EGFP gene (IR-EG) were examined in targeting the EGFP-LqqIT2 (EL)-fusion mRNA or LqqIT2-EGFP (LE)-bicistronic mRNA for degradation. Suppression efficiencies using these inducers were examined transiently and stably in uninfected and infected insect Sf9 cells. Artikel-Nr. 9203388/2
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