Directed Evolution of Selective Enzymes: Catalysts for Organic Chemistry and Biotechnology - Hardcover

Reetz, Manfred T.

 
9783527316601: Directed Evolution of Selective Enzymes: Catalysts for Organic Chemistry and Biotechnology
Hardcover
ISBN 10: 3527316604 ISBN 13: 9783527316601
Verlag: Wiley-VCH, 2016

Inhaltsangabe

Authored by one of the world's leading organic chemists, this authoritative reference provides an overview of basic strategies in directed evolution and introduces common gene mutagenesis, screening and selection methods.
Throughout the text, emphasis is placed on methodology development to maximize efficiency, reliability and speed of the experiments and to provide guidelines for efficient protein engineering. Professor Reetz highlights the application of directed evolution experiments to address limitations in the field of enzyme selectivity, substrate scope, activity and robustness. He critically reviews recent developments and case studies, takes a look at future applications in the field of organic synthesis, and concludes with lessons learned from previous experiments.

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Über die Autorin bzw. den Autor

Manfred T. Reetz is the current Hans-Meerwein-Research-Professor at the University of Marburg (Germany) and former Director and Professor Emeritus of the Max-Planck-Institut für Kohlenforschung in Mülheim (Germany). He started his academic career in the US and returned to Germany for his doctorate, which he received in 1969 from the University of Göttingen. He was Professor at several universities in Germany and the USA and received numerous awards, including the Chirality Medal (2014), Otto Hahn Prize (2011), Tetrahedron Prize for Creativity in Organic Chemistry (2011), Arthur C. Cope Award (2009), Karl Ziegler Prize (2005) and the Leibniz Award of the German Research Council (1989). From 2007 to 2011, Reetz was Senator of the German Academy of Sciences Leopoldina. He has authored more than 550 publications, several book chapters and one book about organotitanium reagents in organic synthesis.

Von der hinteren Coverseite

Authored by one of the world's leading organic chemists and protein engineers, this authoritative monograph provides an overview of basic strategies in directed evolution of enzymes as selective catalysts in organic chemistry and biotechnology. In introductory chapters, Manfred T. Reetz describes the most important gene mutagenesis methods and high-throughput screening and selection techniques, and continues with in-depth analyses of different strategies in laboratory evolution that have emerged in recent years.
Throughout the text, emphasis is placed on methodology development in the quest to maximize efficiency, reliability and speed of directed evolution as a prolific source of robust biocatalysts for asymmetric transformations. Practical guidelines and tips regarding possible pitfalls are provided. The author critically reviews recent developments and case studies, takes a look at future applications in organic synthesis and industrial biotechnology, and concludes with a discussion of the lessons learned from directed evolution.

Aus dem Klappentext

Authored by one of the world's leading organic chemists and protein engineers, this authoritative monograph provides an overview of basic strategies in directed evolution of enzymes as selective catalysts in organic chemistry and biotechnology. In introductory chapters, Manfred T. Reetz describes the most important gene mutagenesis methods and high-throughput screening and selection techniques, and continues with in-depth analyses of different strategies in laboratory evolution that have emerged in recent years.
Throughout the text, emphasis is placed on methodology development in the quest to maximize efficiency, reliability and speed of directed evolution as a prolific source of robust biocatalysts for asymmetric transformations. Practical guidelines and tips regarding possible pitfalls are provided. The author critically reviews recent developments and case studies, takes a look at future applications in organic synthesis and industrial biotechnology, and concludes with a discussion of the lessons learned from directed evolution.

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Verlag
Wiley-VCH
Erscheinungsdatum
2016
Sprache
Englisch
ISBN 10 
3527316604
ISBN 13 
9783527316601
Einband
Gebundene Ausgabe
Auflage
1
Anzahl der Seiten
320
Kontakt zum Hersteller
Wiley-VCH GmbH
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