The Advanced Methods series is intented for advanced undergraduates, postgraduates and established research scientists. Titles in the series are designed to cover current important areas of research in life sciences, and include both theoretical background and detailed protocols. The aim is to give researchers sufficient theory, supported by references, to take the given protocols and adapt them to their particular experimental systems. Unbiased Stereology, second edition expands the comprehensive practical first edition guide to 3-D measurements in microscopy using stereological methods by adding a section on 2-D stereology, or "Petri-metrics". There is also an additional chapter on single object stereology, which permits the measurement of total volume, surface and feature length of single arbitrary objects. The book is intended to lead the reader logically through a self-teaching course, with illustrative exercises at each stage. The techniques described have become benchmark methods in toxicology and neuroscience; the use of inferior methods often leading to missed or spurious results.Reseña del editor:
Unbiased Stereology, Second Edition is a practical guide to making unbiased 3-D measurements via the microscope. Only those stereological techniques which have been tried and tested by real application are included. Although this technology is essentially mathematical and statistical, the authors do not immerse the reader in complex analysis, but rather provide simple heuristic explanations and references to the original proof, and illustrate the theory by analogies drawn from everyday experience.
To give practical experience in application of the techniques, exercises are provided at the end of each chapter, complete with detailed worked answers.
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