Polyamine Protocols (Methods in Molecular Biology, Band 79) - Softcover

 
9781617370427: Polyamine Protocols (Methods in Molecular Biology, Band 79)
Softcover
ISBN 10: 1617370428 ISBN 13: 9781617370427
Verlag: Humana, 2010

Inhaltsangabe

Polyamines: An Introduction.- Assay Methods for Enzymes of Polyamine Biosynthesis.- Determination of Ornithine Decarboxylase Activity Using [3H]Omithine.- Assay of Mammalian Ornithine Decarboxylase Activity Using [14C]Ornithine.- Assay of Mammalian S-Adenosylmethionine Decarboxylase Activity.- Assay of Spermidine and Spermine Synthases.- Measurement of Spermidine/Spermine N 1-Acetyltransferase Activity.- Assay of Spermidine N 8-Acetyltransferase.- Assay Methods for Enzymes of Polyamine Catabolism.- Assay of N 8-Acetylspermidine Deacetylase.- Hydrogen Peroxide Assay for Amine Oxidase Activity.- Radiochemical Assay of Diamine Oxidase.- Radiochemical Estimation of Polyamine Oxidase.- Measurement of Aldehyde Formation as an Assay for Polyamine Oxidase Activity.- Measurement of Polyamines.- Determination of Polyamines as Their Benzoylated Derivatives by HPLC.- A Dansyl Chloride-HPLC Method for the Determination of Polyamines.- The Determination of Polyamines and Amino Acids by a Fluorescamine-HPLC Method.- Thin-Layer Chromatographic Method for Assaying Polyamines.- Measurement of Polyamine Transport.- Measurement of Polyamine Transport: Adherent Cells.- Measurement of Polyamine Transport: Cells in Suspension.- Measurement of Polyamine Efflux from Cells in Culture.- Measurement of Polyamine Effects on Cell Growth.- Leucine Incorporation and Thymidine Incorporation.- Tetrazolium (MTT) Assay for Cellular Viability and Activity.

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David Morgan brings together in Polyamine Protocols a unique collection of hands-on enzyme assay techniques for the study of polyamines and their biological functions. The techniques range from assay methods for enzymes of polyamine biosynthesis and catabolism to measurements of polyamines, polyamine transport, and polyamine effects on cell growth. All methods are presented by active researchers who have developed them to a high degree of perfection in their own laboratories, and include clear, step-by-step instructions with numerous hints and tips to ensure readily reproducible results.

Polyamine Protocols is the only compilation of methods currently available for use in studies involving polyamines. The collection offers both novice and experienced researchers across many fields-including cell and molecular biology, pharmacology, oncology, physiology, laboratory medicine, and clinical pathology-all the tools needed to elucidate the biological roles played by these ubiquitous substances.

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Verlag
Humana
Erscheinungsdatum
2010
Sprache
Englisch
ISBN 10 
1617370428
ISBN 13 
9781617370427
Einband
Taschenbuch
Anzahl der Seiten
200
Herausgeber
Morgan David M. L.
Kontakt zum Hersteller
preigu GmbH & Co. KG
mail@preigu.de

Lengericher Landstr. 19
Osnabrück
Niedersachsen
49078
Deutschland

Weitere beliebte Ausgaben desselben Titels

9780896034488: Polyamine Protocols (Methods in Molecular Biology, 79, Band 79)

Vorgestellte Ausgabe

ISBN 10:  0896034488 ISBN 13:  9780896034488
Verlag: Humana, 1997
Hardcover