Verwandte Artikel zu Differential Display Methods and Protocols: 317 (Methods...

Differential Display Methods and Protocols: 317 (Methods in Molecular Biology) - Hardcover

 
9781588293381: Differential Display Methods and Protocols: 317 (Methods in Molecular Biology)

Inhaltsangabe

Since the first edition of this book dedicated to differential display (DD) technology was published in 1997, we have witnessed an explosive interest in studying differential gene expression. The gene-hunting euphoria was initially powered by the invention of DD, which was gradually overtaken by DNA microarray technology in recent years. Then why is there still the need for second edition of this DD book? First of all, DD still enjoys a substantial lead over DNA microarrays in the ISI citation data (see Table 1), despite the h- dreds of millions of dollars spent each year on arrays. This may come as a surprise to many, but to us it implies that many of the DNA microarray studies went unpublished owing to their unfulfilled promises (1). Second, unlike DNA microarrays, DD is an "open"-ended gene discovery method that does not depend on prior genome sequence information of the organism being studied. As such, DD is applicable to the study of all living organisms-from bacteria, fungi, insects, fish, plants, to mammals-even when their genomes are not sequenced. Second, DD is more accessible technically and financially to most cost-conscious "cottage-industry" academic laboratories. So clearly DD still has its unique place in the modern molecular biological toolbox for gene expression analysis.

Die Inhaltsangabe kann sich auf eine andere Ausgabe dieses Titels beziehen.

Von der hinteren Coverseite

Carrying on the high standards of the first edition of Differential Display Methods, Peng Liang et al. have based their second edition on a new mathematical model of differential display (DD) that takes advantage of automation, as well as digital data acquisition and analysis. These well-versed authors explain and highlight all the recent methodological refinements, including automated liquid handling of hundreds of DD PCR reaction setups combined with capillary electrophoresis, a prototype computer program to automatically allow positive band identification from a fluorescence differential display image, and restriction fragment-based DD screenings that can link any cDNA fragment directly to a given gene once the sequence information of all transcripts becomes available. Other improvements discussed are combining DD and DNA microarrays by reducing the complexity of cDNA probes while increasing the sensitivity of detection, and a DD approach to detect prokaryotic mRNA expression. The authors also demonstrate the power of DD technology with a collection of outstanding examples of DD applications and detailed experimental procedures. The elegant studies described here have led to the discovery of many important genes involved in viral infection, Prion disease, cancer, ovulation, circadian clock, floral color, transcription repression gene silencing, mRNA polymorphism, and protein-RNA interaction.
State-of-the-art and highly practical, Differential Display Methods, Second Edition offers gene hunters the possibility of genome-wide comprehensive DD screening, as well as a proven road map for any successful "gene fishing" expedition.

„Über diesen Titel“ kann sich auf eine andere Ausgabe dieses Titels beziehen.

Gebraucht kaufen

Zustand: Befriedigend
Ex- library copy with stamps and...
Diesen Artikel anzeigen

EUR 12,68 für den Versand von USA nach Deutschland

Versandziele, Kosten & Dauer

Gratis für den Versand innerhalb von/der Deutschland

Versandziele, Kosten & Dauer

Weitere beliebte Ausgaben desselben Titels

9781617375033: Differential Display Methods and Protocols: 317 (Methods in Molecular Biology)

Vorgestellte Ausgabe

ISBN 10:  1617375039 ISBN 13:  9781617375033
Verlag: Humana, 2010
Softcover

Suchergebnisse für Differential Display Methods and Protocols: 317 (Methods...

Beispielbild für diese ISBN

Verlag: Humana, 2005
ISBN 10: 1588293386 ISBN 13: 9781588293381
Gebraucht Hardcover

Anbieter: Books From California, Simi Valley, CA, USA

Verkäuferbewertung 4 von 5 Sternen 4 Sterne, Erfahren Sie mehr über Verkäufer-Bewertungen

hardcover. Zustand: Good. Ex- library copy with stamps and stickers. Artikel-Nr. mon0003287673

Verkäufer kontaktieren

Gebraucht kaufen

EUR 33,85
Währung umrechnen
Versand: EUR 12,68
Von USA nach Deutschland
Versandziele, Kosten & Dauer

Anzahl: 1 verfügbar

In den Warenkorb

Foto des Verkäufers

Peng Liang
ISBN 10: 1588293386 ISBN 13: 9781588293381
Neu Hardcover Erstausgabe

Anbieter: buchversandmimpf2000, Emtmannsberg, BAYE, Deutschland

Verkäuferbewertung 5 von 5 Sternen 5 Sterne, Erfahren Sie mehr über Verkäufer-Bewertungen

Buch. Zustand: Neu. Neuware -Since the first edition of this book dedicated to differential display (DD) technology was published in 1997, we have witnessed an explosive interest in studying differential gene expression. The gene-hunting euphoria was initially powered by the invention of DD, which was gradually overtaken by DNA microarray technology in recent years. Then why is there still the need for second edition of this DD book First of all, DD still enjoys a substantial lead over DNA microarrays in the ISI citation data (see Table 1), despite the h- dreds of millions of dollars spent each year on arrays. This may come as a surprise to many, but to us it implies that many of the DNA microarray studies went unpublished owing to their unfulfilled promises (1). Second, unlike DNA microarrays, DD is an ¿open¿-ended gene discovery method that does not depend on prior genome sequence information of the organism being studied. As such, DD is applicable to the study of all living organisms¿from bacteria, fungi, insects, fish, plants, to mammals¿even when their genomes are not sequenced. Second, DD is more accessible technically and financially to most cost-conscious ¿cottage-industry¿ academic laboratories. So clearly DD still has its unique place in the modern molecular biological toolbox for gene expression analysis.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 336 pp. Englisch. Artikel-Nr. 9781588293381

Verkäufer kontaktieren

Neu kaufen

EUR 106,99
Währung umrechnen
Versand: Gratis
Innerhalb Deutschlands
Versandziele, Kosten & Dauer

Anzahl: 2 verfügbar

In den Warenkorb

Beispielbild für diese ISBN

Verlag: Humana, 2005
ISBN 10: 1588293386 ISBN 13: 9781588293381
Neu Hardcover

Anbieter: Ria Christie Collections, Uxbridge, Vereinigtes Königreich

Verkäuferbewertung 5 von 5 Sternen 5 Sterne, Erfahren Sie mehr über Verkäufer-Bewertungen

Zustand: New. In. Artikel-Nr. ria9781588293381_new

Verkäufer kontaktieren

Neu kaufen

EUR 118,75
Währung umrechnen
Versand: EUR 5,88
Von Vereinigtes Königreich nach Deutschland
Versandziele, Kosten & Dauer

Anzahl: Mehr als 20 verfügbar

In den Warenkorb

Beispielbild für diese ISBN

Liang, Peng (Editor)/ Meade, Jonathan D. (Editor)/ Pardee, Arthur B. (Editor)
Verlag: Humana Pr Inc, 2005
ISBN 10: 1588293386 ISBN 13: 9781588293381
Neu Hardcover

Anbieter: Revaluation Books, Exeter, Vereinigtes Königreich

Verkäuferbewertung 5 von 5 Sternen 5 Sterne, Erfahren Sie mehr über Verkäufer-Bewertungen

Hardcover. Zustand: Brand New. 2nd edition. 336 pages. 9.00x6.00x1.00 inches. In Stock. Artikel-Nr. x-1588293386

Verkäufer kontaktieren

Neu kaufen

EUR 163,81
Währung umrechnen
Versand: EUR 11,81
Von Vereinigtes Königreich nach Deutschland
Versandziele, Kosten & Dauer

Anzahl: 2 verfügbar

In den Warenkorb