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Inhaltsangabe
This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and invaluable, CRISPR Gene Editing: Methods and Protocols will assist undergraduates, graduates, and researchers with detailed guidelines and methods for the vitally important CRISPR gene editing field.
Chapter 3 is available open access under a CC BY 4.0 license via link.springer.com.
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This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and invaluable, CRISPR Gene Editing: Methods and Protocols will assist undergraduates, graduates, and researchers with detailed guidelines and methods for the vitally important CRISPR gene editing field.
Chapter 3 is available open access under a CC BY 4.0 license via link.springer.com.
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- VerlagHumana
- Erscheinungsdatum2019
- ISBN 10 1493991698
- ISBN 13 9781493991693
- EinbandTapa dura
- SpracheEnglisch
- Auflage1
- Anzahl der Seiten376
- HerausgeberLuo Yonglun
- Kontakt zum HerstellerNicht verfügbar
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CRISPR Gene Editing
Verlag:
Springer New York, Springer US Mär 2019, 2019
ISBN 10: 1493991698
ISBN 13: 9781493991693
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Hardcover
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Buch. Zustand: Neu. Neuware -This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 376 pp. Englisch. Artikel-Nr. 9781493991693
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CRISPR Gene Editing : Methods and Protocols
Verlag:
Springer New York, Springer New York, 2019
ISBN 10: 1493991698
ISBN 13: 9781493991693
Neu
Hardcover
Anbieter: AHA-BUCH GmbH, Einbeck, Deutschland
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Buch. Zustand: Neu. Druck auf Anfrage Neuware - Printed after ordering. Artikel-Nr. 9781493991693
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CRISPR Gene Editing: Methods and Protocols (Methods in Molecular Biology, 1961)
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Crispr Gene Editing: Methods and Protocols
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Hardcover. Zustand: Brand New. 362 pages. 10.00x7.00x1.00 inches. In Stock. Artikel-Nr. x-1493991698
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