The CRAC Channel: Methods and Protocols (Methods in Molecular Biology, 1843, Band 1843) - Hardcover

 
9781493987023: The CRAC Channel: Methods and Protocols (Methods in Molecular Biology, 1843, Band 1843)
Hardcover
ISBN 10: 149398702X ISBN 13: 9781493987023
Verlag: Humana, 2018

Inhaltsangabe

This volume discusses the cellular and molecular techniques used to study and characterize the different components of the CRAC channel signaling pathway. The chapters in this book cover topics such as fluorescence-based measurements of the CRAC channel activity in cell populations; patch-clamp recording of the CRAC channel in STIM-Orai overexpressing cells and native systems; western-blotting and co-immunoprecipitation of endogenous STIM/ORAI and protein partners; shRNA-mediated gene silencing; and the SC-SMD system. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, The CRAC Channel: Methods and Protocols is a valuable resource for any researcher interested in learningmore about the CRAC channel, as its activation mechanism and roles in cellular functions have yet to be completely explored.

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This volume discusses the cellular and molecular techniques used to study and characterize the different components of the CRAC channel signaling pathway. The chapters in this book cover topics such as fluorescence-based measurements of the CRAC channel activity in cell populations; patch-clamp recording of the CRAC channel in STIM-Orai overexpressing cells and native systems; western-blotting and co-immunoprecipitation of endogenous STIM/ORAI and protein partners; shRNA-mediated gene silencing; and the SC-SMD system. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, The CRAC Channel: Methods and Protocols is a valuable resource for any researcher interested in learning more about the CRAC channel, as its activation mechanism and roles in cellular functions have yet to be completely explored.

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Verlag
Humana
Erscheinungsdatum
2018
Sprache
Englisch
ISBN 10 
149398702X
ISBN 13 
9781493987023
Einband
Gebundene Ausgabe
Auflage
1
Anzahl der Seiten
215
Herausgeber
Penna Aubin, Constantin Bruno
Kontakt zum Hersteller
Springer Nature Customer Service Center GmbH
ProductSafety@springernature.com

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Heidelberg
69115
Deutschland

Weitere beliebte Ausgaben desselben Titels

9781493993703: The CRAC Channel: Methods and Protocols (Methods in Molecular Biology, Band 1843)

Vorgestellte Ausgabe

ISBN 10:  1493993704 ISBN 13:  9781493993703
Verlag: Humana, 2019
Softcover