Membrane Proteins Production for Structural Analysis

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9781493948062: Membrane Proteins Production for Structural Analysis
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This book updates the latest development in production, stabilization and structural analysis techniques of membrane proteins. This field has made significant advances since the elucidation of the first 3-D structure of a recombinant G Protein Coupled Receptor (GPCR), rhodopsin, with the structure of several more GPCRs having been solved in the past five years. In fact, the 2012 Nobel Prize in Chemistry was awarded for groundbreaking discoveries on the inner workings of GPCRs. This book is essential reading for all researchers, biochemists and crystallographers working with membrane proteins, who are interested by the structural characterization of their favorite protein and who wish to follow the expression, migration, modifications and recycling of a membrane protein.


This volume reviews the latest development in production, stabilization and structural analysis techniques of membrane proteins. It contains 14 chapters exploring topics including the advances in heterologous expression systems, stabilization tools and structural methods that contributed to the growing number of recombinant integral membrane protein structures solved in the past few years. Each chapter was written by internationally renowned scientists in the field of membrane proteins structural characterization.

Membrane proteins account for roughly 30 percent of all open reading frames in fully sequenced genomes. However, to date, atomic structures have so far been obtained for only 424 integral membrane proteins, with 100 new structures determined in the last two years. Only 10 percent of the unique integral membrane protein structures are derived from vertebrates. In general, integral membrane proteins are present in tissues at very low concentration, making production of recombinant proteins in heterologous systems suitable for large scale production a prerequisite for structural studies. Since the first atomic structures of recombinant mammalian integral membrane proteins published in 2005 (the calcium ATPase SERCA 1A and a voltage-dependent potatium channel), the structures of 37 recombinant mammalian integral membrane proteins, from which 20 belong to the G Protein Coupled Receptors family, have been solved.

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9781493906611: Membrane Proteins Production for Structural Analysis

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ISBN 10:  1493906615 ISBN 13:  9781493906611
Verlag: Springer, 2014