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<P>THIS VOLUME PROVIDES READERS WITH A COMPREHENSIVE REVIEW OF VARIOUS GENE SILENCING METHODOLOGIES AND ITS APPLICATIONS. IT PROVIDES A HISTORICAL OVERVIEW OF GENE SILENCING MECHANISMS IN PLANTS, VECTORS, AND STRATEGIES AVAILABLE FOR PLANT GENE SILENCING, PRACTICAL APPLICATIONS OF GENE SILENCING, BIOINFORMATICS TOOLS, AND OTHER RESOURCES. IN ADDITION TO THESE REVIEW CHAPTERS, THIS BOOK INCLUDES METHODOLOGY FOR VIRUS-INDUCED GENE SILENCING (VIGS) IN VARIOUS DIFFERENT PLANT SPECIES, UNDERSTANDING PLANT STRESS RESPONSES USING VIGS, MIRNA IDENTIFICATION, DNA INTERFERENCE, HOST-INDUCED GENE SILENCING, USE OF ARTIFICIAL MIRNAS FOR GENE SILENCING, HIGH THROUGHPUT RNAI, AND MORE. WRITTEN IN THE HIGHLY SUCCESSFUL <I>METHODS OF MOLECULAR BIOLOGY</I> SERIES FORMAT, CHAPTERS INCLUDE INTRODUCTIONS TO THEIR RESPECTIVE TOPICS, LISTS OF THE NECESSARY MATERIALS AND REAGENTS, STEP-BY-STEP, READILY REPRODUCIBLE LABORATORY PROTOCOLS, AND KEY TIPS ON TROUBLESHOOTING AND AVOIDING KNOWN PITFALLS.</P><P>THOROUGH AND PRACTICAL, <I>PLANT GENE SILENCING: METHODS AND PROTOCOLS</I> SEEKS TO AID SCIENTISTS TO FURTHER UNDERSTAND FUNCTIONAL RELEVANCE OF TARGET GENES USING GENE SILENCING METHODS AND USE THESE TECHNOLOGIES IN COMMERCIAL PLANT VARIETIES.</P>
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This volume provides readers with a comprehensive review of various gene silencing methodologies and its applications. It provides a historical overview of gene silencing mechanisms in plants, vectors, and strategies available for plant gene silencing, practical applications of gene silencing, bioinformatics tools, and other resources. In addition to these review chapters, this book includes methodology for virus-induced gene silencing (VIGS) in various different plant species, understanding plant stress responses using VIGS, miRNA identification, DNA interference, host-induced gene silencing, use of artificial miRNAs for gene silencing, high throughput RNAi, and more. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Thorough and practical, Plant Gene Silencing: Methods and Protocols seeks to aid scientists to further understand functional relevance of target genes using gene silencing methods and use these technologies in commercial plant varieties.
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- VerlagHumana Press
- Erscheinungsdatum2015
- ISBN 10 1493924524
- ISBN 13 9781493924523
- EinbandTapa dura
- SpracheEnglisch
- Anzahl der Seiten320
- HerausgeberMysore Kirankumar S., Senthil-Kumar Muthappa
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Plant Gene Silencing: Methods and Protocols (Methods in Molecular Biology, 1287)
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Plant Gene Silencing : Methods and Protocols
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Springer New York, Springer New York, 2015
ISBN 10: 1493924524
ISBN 13: 9781493924523
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Buch. Zustand: Neu. Druck auf Anfrage Neuware - Printed after ordering - This volume provides readers with a comprehensive review of various gene silencing methodologies and its applications. It provides a historical overview of gene silencing mechanisms in plants, vectors, and strategies available for plant gene silencing, practical applications of gene silencing, bioinformatics tools, and other resources. In addition to these review chapters, this book includes methodology for virus-induced gene silencing (VIGS) in various different plant species, understanding plant stress responses using VIGS, miRNA identification, DNA interference, host-induced gene silencing, use of artificial miRNAs for gene silencing, high throughput RNAi, and more. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Thorough and practical, Plant Gene Silencing: Methods and Protocols seeks to aid scientists to further understand functional relevance of target genes using gene silencing methods and use these technologies in commercial plant varieties. Artikel-Nr. 9781493924523
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