Dissertation: I. The Detection and Determination of Minute Quantities of Glycerine, II. The Volumes of Weight-Normal Cane Sugar Solutions at Different ... of the Johns Hopkins University in Conformity - Softcover

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Excerpt from Dissertation: I. The Detection and Determination of Minute Quantities of Glycerine, II. The Volumes of Weight-Normal Cane Sugar Solutions at Different Temperatures; Submitted to the Board of University Studies of the Johns Hopkins University in Conformity With the Requ

The Detection and Determination of Minute Quantities of Glycerine.

In the measurement of osmotic pressure in this laboratory, no measurement is regarded as conclusive until it has been shown that the membrane was not broken, thereby allowing some of the solution to escape from the cell. In other words, the solution whose osmotic pressure is being measured must have the same concentration after the cell has been up days, or in some cases weeks, as it had when it was first put up. There are two ways of solving this problem. Either the solu tion on the inside of the cell must be tested before and after the experiment, to ascertain whether or not any dilution has taken place, or the solution on the outside of the cell must be tested to ascertain whether or not it contains any of the substance whose osmotic pressure is being measured.

The polarimeter affords an excellent method for substances which are optically active, but in the case of glycerine, it was thought more practicable to test the water in which the cell is immersed for the presence of this substance. It was necessary to search for some methods for the detection and determination of minute quantities of this substance.

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