The Journal of Comparative Neurology (Volume 7) - Softcover

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This historic book may have numerous typos and missing text. Purchasers can download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated.1897 Excerpt: ... In the same ratio there are no empty spaces left about the cell body. In the specimens fixed with alcohol-formalin one is struck at first sight by the variation in the staining, these sections taking up methylene blue as well as thionine blue quite strongly, more so than the preceding. In the ganglion cells the body is separated from the surrounding structures slightly; whether this is due to its contraction or the contraction of the surrounding tissues away from it, could not be definitely determined. The granules are slightly indistinct, poorer than with specimens preserved with absolute alcohol. With formalin 20% the staining was even more intense than with the preceding and the contraction of or away from the ganglion cell body was marked. Yet the chromophilic granules came out distinctly. Fixation with Hermann's fluid gave the worst results. The contraction was uniform throughout but the granular structure was irregular, lumpy or broken up into fine granular masses. Flemming's fluid gave better results, the chromophilic granules being often quite sharp and well preserved; contraction was marked, as with the Hermann's. Thus alcohol 95 % and sat. alcoholic sol. of corrosive sublimate gave the sharpest pictures. As to the use of thionine blue, instead of methylene blue, the comparisons made were in favor of the methylene blue. With reference to the mounting media, I cannot feel that there is much preference to be shown. Nissl highly recommended benzine colophonium but in the experiments made I could not say that I could detect any differences in the media used, and on submission of the slides to Dr. Strong he could not tell that there was any difference. Perhaps after a lapse of some time there may be differences. Benzine colophonium dries very rapidly an...

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