Directed Evolution: Methods and Protocols: 2461 (Methods in Molecular Biology) - Hardcover

Buch 109 von 274: Methods in Molecular Biology
 
9781071621516: Directed Evolution: Methods and Protocols: 2461 (Methods in Molecular Biology)

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This volume explores the latest techniques used by researchers to study directed evolution (DE) at each stage of the Design-Build-Test-Learn cycle. Chapters in this book cover topics such as designing overlap extension PCR primers for protein mutagenesis; antha-guided automation of Darwin assembly for the construction of bespoke gene libraries; rapid cloning of random mutagenesis libraries using PTO-Quickstep; and DE of glycosyltransferases by a single-cell screening method. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cutting-edge and comprehensive, Directed Evolution: Methods and Protocols is a valuable resource for scientists and researchers who are interested in learning more about this field and incorporating these studies into new experimental workflows.


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This volume explores the latest techniques used by researchers to study directed evolution (DE) at each stage of the Design-Build-Test-Learn cycle. Chapters in this book cover topics such as designing overlap extension PCR primers for protein mutagenesis; antha-guided automation of Darwin assembly for the construction of bespoke gene libraries; rapid cloning of random mutagenesis libraries using PTO-Quickstep; and DE of glycosyltransferases by a single-cell screening method. Written in the highly successful <i>Methods in Molecular Biology</i> series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.&nbsp;<br><div>Cutting-edge and comprehensive, <i>Directed Evolution: Methods and Protocols</i> is a valuable resource for scientists and researchers who are interested in learning more about this field and incorporating these studies into new experimental workflows.</div><div><br></div>

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9781071621547: Directed Evolution: Methods and Protocols: 2461 (Methods in Molecular Biology)

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ISBN 10:  1071621548 ISBN 13:  9781071621547
Verlag: Humana, 2023
Softcover