Mycobacterium ulcerans: Methods and Protocols: 2387 (Methods in Molecular Biology) - Softcover

 
9781071617816: Mycobacterium ulcerans: Methods and Protocols: 2387 (Methods in Molecular Biology)
Softcover
ISBN 10: 1071617818 ISBN 13: 9781071617816
Verlag: Humana, 2022

Inhaltsangabe

Preface...
Table of Contents...
Contributing Authors...

Part I Mycobacterium ulcerans Disease and Host Pathogen Interaction

1. Overview: Mycobacterium ulcerans Disease (Buruli ulcer)
Katharina Röltgen and Gerd Pluschke

2. Immunohistochemistry: A Method to Analyze M.ulcerans Infected Skin Tissue
Marie-Thérèse Ruf

3. Primary Isolation of Mycobacterium ulcerans
Dorothy Yeboah-Manu, Adwoa Asante-Poku, and Samuel Yaw Aboagye

4. Investigation of Mycobacterium ulcerans Glycan Interactions Using Glycan Array and Surface Plasmon Resonance
Christopher J. Day, Jessica Poole, Gerd Pluschke, and Michael P. Jennings

5. Purification and Characterization of Extracellular Vesicles from Mycobacterium ulcerans Culture
Mélanie Foulon, Estelle Marion, and Laurent Marsollier

6. DNA Extraction from Clinical Specimens for the Direct Detection of Mycobacterium ulcerans by Real-Time PCR
Caroline J. Lavender and Janet A.M. Fyfe

7. Extraction of DNA from Environmental Samples for the Real-Time PCR Detection of Mycobacterium ulcerans
Janet A.M. Fyfe and Caroline J. Lavender

8. Detection of Mycobacterium ulcerans DNA using Real-Time PCR
 Janet A.M. Fyfe and Caroline J. Lavender

9. Determining Viability of M. ulcerans by 16S rRNA RT Reverse Transcriptase Real-Time PCR
Marcus Beissner, Richard Odame Phillips, and Gisela Bretzel

10. Methods and Approaches for Buruli ulcer Surveillance in Africa: Lessons Learnt and Future Directions
Joseph W. S. Timothy, Rachel L. Pullan, and Rie R. Yotsu


Part II Purification and Quantification of Myolactone 

11. Overview: Mycolactone, The Macrolide Toxin of Mycobacterium ulcerans
Katharina Röltgen and Gerd Pluschke

12. Conditions for Handling and Optimal Storage of Mycolactone
Jessica Z. Kubicek-Sutherland, Basil I. Swanson, and Harshini Mukundan

13. Mycolactone Purification from M. ulcerans Cultures and HPLC-Based Approaches for Mycolactone Quantification in Biological Samples 
Aline Rifflet, Caroline Demangel, and Laure Guenin-Macé

14. Detection of Mycolactone by Thin Layer Chromatography
Richard Kwamla Amewu and Thomas Spangenberg

15. Competitive ELISA for the Detection and Quantification of Mycobacterium ulcerans Mycolactone
Louisa Warryn

16. Biochemical and Biological Assays of Mycolactone-Mediated Inhibition of Sec61
Sarah O'Keefe, Stephen High, and Caroline Demangel

Part III Drug Development against Mycobacterium ulcerans

17. Overview: Development of Drugs against Mycobacterium ulcerans
Gerd Pluschke and Katharina Röltgen

18. In Vitro Assessment of Drug Activity against Mycobacterium ulcerans
Oliver D. Komm, Deepak V. Almeida, Paul J. Converse, and Eric L. Nuermberger

19. Drug Efficacy Testing in the Mouse Footpad Model of Buruli ulcer
Paul J. Converse, Deepak V. Almeida, and Eric L. Nuermberger

20. Turbidity-Based MIC Assay and Characterization of Spontaneous Drug Resistant Mutants in Mycobacterium ulcerans
Sangeeta Susan Thomas, Nitin Pal Kalia, and Kevin Pethe

21. Determination of Bioenergetic Parameters in Mycobacterium ulcerans
Sangeeta Susan Thomas and Kevin Pethe

Subject Index List...

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Von der hinteren Coverseite

This volume explores the latest techniques used to study Mycobacterium ulcerans, and more specifically M. ulcerans disease (Buruli ulcer). The chapters in this book are organized into three parts and cover methods for the detection of M. ulcerans and the analysis of host-pathogen interaction; the quantification and characterization of mycolactone, the macrolide toxin of M. ulcerans; and drug development against M. ulcerans. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Mycobacterium ulcerans: Methods and Protocols is a valuable resource that helps scientists advance their research on Buruli ulcer, which is still an under-researched field in infection biology.

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Verlag
Humana
Erscheinungsdatum
2022
Sprache
Englisch
ISBN 10 
1071617818
ISBN 13 
9781071617816
Einband
Tapa blanda
Auflage
1
Anzahl der Seiten
244
Herausgeber
Pluschke Gerd, Röltgen Katharina
Kontakt zum Hersteller
Nicht verfügbar
Verantwortliche Person
Nicht verfügbar

Weitere beliebte Ausgaben desselben Titels

9781071617786: Mycobacterium ulcerans: Methods and Protocols: 2387 (Methods in Molecular Biology)

Vorgestellte Ausgabe

ISBN 10:  1071617788 ISBN 13:  9781071617786
Verlag: Springer-Verlag GmbH, 2021
Hardcover