Cell Cycle Checkpoints: Methods and Protocols: 2267 (Methods in Molecular Biology) - Softcover

 
9781071612194: Cell Cycle Checkpoints: Methods and Protocols: 2267 (Methods in Molecular Biology)
Softcover
ISBN 10: 1071612190 ISBN 13: 9781071612194
Verlag: Humana, 2022

Inhaltsangabe

Preface...
Table of Contents...
Contributing Authors..

1. Determination of CHK1 Cellular Localization by Immunofluorescence Microscopy
Yu-Che Cheng and Sheau-Yann Shieh

2. Detection of Post-Translationally Modified p53 by Western Blotting
Anna Estevan Barber and David W. Meek

3. Global Analyses to Identify Direct Transcriptional Targets of p53
Matthew D. Galbraith, Zdenek Andrysik, Kelly D. Sullivan, and Joaquín M. Espinosa

4. Analysis of Replication Dynamics Using the Single-Molecule DNA Fiber Spreading Assay
Stephanie Biber and Lisa Wiesmüller

5. Measuring Translation Efficiency by RNA Immunoprecipitation of Translation Initiation Factors
Chris Lucchesi, Shakur Mohibi, and Xinbin Chen

6. DNA Affinity Purification: A Pulldown Assay for Identifying and Analyzing Proteins Binding to Nucleic Acids
Gerd A. Müller and Kurt Engeland

7. A Novel Strategy to Track Lysine-48 Ubiquitination by Fluorescence Resonance Energy Transfer
Kenneth Wu and Zhen-Qiang Pan

8. The DNA Damage Response in Xenopus laevis Cell-Free Extracts
Tomas Aparicio Casado and Jean Gautier

9. Mammalian Cell Fusion Assays for the Study of Cell Cycle Progression by Functional Complementation
Jongkuen Lee and David Dominguez-Siola

10. Knockdown of Target Genes by siRNA In Vitro
Songhee Back and James J. Manfredi

11. Assaying Cell Cycle Status Using Flow Cytometry
Ramy Rahme

12. Using TUNEL Assay to Quantitate p53-Induced Apoptosis in Mouse Tissues
Lois Resnick-Silverman

13. Generation and Analysis of dsDNA Breaks for Checkpoint and Repair Studies in Fission Yeast
Rohana Ramalingam and Matthew J. O'Connell

14. Calreticulin Exposure in Mitotic Catastrophe
Lucillia Bezu, Oliver Kepp, and Guido Kroemer

15. Quantification of eIF2α Phosphorylation Associated with Mitotic Catastrophe by Immunofluorescence Microscopy
Juliette Humeau, Lucillia Bezu, Oliver Kepp, Laura Senovilla, Peng Liu, and Guido Kroemer

16. Clonogenic Assays to Detect Cell Fate in Mitotic Catastrophe
José Manuel Bravo-San Pedro, Oliver Kepp, Allan Sauvat, Santiago Rello-Varona, Guido Kroemer, and Laura Senovilla

Subject Index List...

Die Inhaltsangabe kann sich auf eine andere Ausgabe dieses Titels beziehen.

Über die Autorin bzw. den Autor

Charlotte y Peter Fiell son dos autoridades en historia, teoría y crítica del diseño y han escrito más de sesenta libros sobre la materia, muchos de los cuales se han convertido en éxitos de ventas. También han impartido conferencias y cursos como profesores invitados, han comisariado exposiciones y asesorado a fabricantes, museos, salas de subastas y grandes coleccionistas privados de todo el mundo. Los Fiell han escrito numerosos libros para TASCHEN, entre los que se incluyen 1000 Chairs, Diseño del siglo XX, El diseño industrial de la A a la Z, Scandinavian Design y Diseño del siglo XXI.

Von der hinteren Coverseite

This volume explores the latest advancements in the field of cell cycle checkpoints and their implications for human diseases. Chapters in this book cover topics such as post-translationally modified p53 by western blotting; CHK1 cellular localization by immunofluorescence microscopy; DNA affinity purification; knockdown of target genes by siRNA in vitro; and calreticulin exposure in mitotic catastrophe. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Cell Cycle Checkpoints: Methods and Protocols is a valuable resource for researcher interested in learning more about this developing field.

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Verlag
Humana
Erscheinungsdatum
2022
Sprache
Englisch
ISBN 10 
1071612190
ISBN 13 
9781071612194
Einband
Tapa blanda
Auflage
1
Anzahl der Seiten
256
Herausgeber
Manfredi James J.
Kontakt zum Hersteller
Nicht verfügbar
Verantwortliche Person
Nicht verfügbar

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Vorgestellte Ausgabe

ISBN 10:  1071612166 ISBN 13:  9781071612163
Hardcover