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CRISPR Guide RNA Design: Methods and Protocols (Methods in Molecular Biology, 2162, Band 2162) - Hardcover

 
9781071606865: CRISPR Guide RNA Design: Methods and Protocols (Methods in Molecular Biology, 2162, Band 2162)
Hardcover
ISBN 10: 1071606867 ISBN 13: 9781071606865
Verlag: Humana, 2020

Inhaltsangabe

This detailed volume focuses on the CRISPR-associated guide RNA and how it can be designed, modified, and validated for a broad repertoire of purposes. Beginning with a section on computational design of target-specific guide RNAs, the book continues by covering chemical modifications to alter guide RNA stability, specificity, and efficiency, as well as to create inducible guide RNAs, append additional functional domains, and express guide RNAs in a conditional manner. It concludes with methods for measuring off-target guide RNA activity. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 


Authoritative and essential, CRISPR Guide RNA Design: Methods and Protocols provides a comprehensive pipeline for guide RNA design and aims to be an invaluable resource in applying this powerful technology to basic research and therapeutic applications.

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This detailed volume focuses on the CRISPR-associated guide RNA and how it can be designed, modified, and validated for a broad repertoire of purposes. Beginning with a section on computational design of target-specific guide RNAs, the book continues by covering chemical modifications to alter guide RNA stability, specificity, and efficiency, as well as to create inducible guide RNAs, append additional functional domains, and express guide RNAs in a conditional manner. It concludes with methods for measuring off-target guide RNA activity. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 


Authoritative and essential, CRISPR Guide RNA Design: Methods and Protocols provides a comprehensive pipeline for guide RNA design and aims to be an invaluable resource in applying this powerful technology to basic research and therapeutic applications.

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Verlag
Humana
Erscheinungsdatum
2020
Sprache
Englisch
ISBN 10 
1071606867
ISBN 13 
9781071606865
Einband
Gebundene Ausgabe
Auflage
1
Anzahl der Seiten
296
Herausgeber
Fulga Tudor A., Knapp David J. H. F., Ferry Quentin R. V.
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