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CRISPR-Cas Methods (Springer Protocols Handbooks) - Hardcover

Buch 48 von 84: Springer Protocols Handbooks
 
9781071606155: CRISPR-Cas Methods (Springer Protocols Handbooks)
Hardcover
ISBN 10: 1071606158 ISBN 13: 9781071606155
Verlag: Humana, 2020

Inhaltsangabe

This volume details the fundamentals of the CRISPR-Cas system, and its protocols illustrate advances in CRISPR-Cas techniques for efficient genome editing. Introductory chapters provide a wide horizon of CRISPR/Cas-based methods and applications. Additional chapters guide readers through HDR-mediated editing, sgRNA design, the step-by-step procedure of multiplex adenine base editing experiments in rice, generating mutants for rice, wheat, Brachypodium, Barley, Flax, and Phytophthora, visual screening of mutants, gene deletion (knock-out), tagging (knock-in) in mammalian cells, the cloning-free (DNA-free) technique, cell-penetrating peptides, generating a genome-edited banana, and nuclear genome editing of Chlamydomonas employing CRISPR-Cpf1 combined with a single-stranded DNA (ssODN) repair template. 

Authoritative and cutting-edge, CRISPR-Cas Methods aims to assist researchers who are new to the field and are aiming to learn how best to adopt this technology for a particular organism.

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This volume details the fundamentals of the CRISPR-Cas system, and its protocols illustrate advances in CRISPR-Cas techniques for efficient genome editing. Introductory chapters provide a wide horizon of CRISPR/Cas-based methods and applications. Additional chapters guide readers through HDR-mediated editing, sgRNA design, the step-by-step procedure of multiplex adenine base editing experiments in rice, generating mutants for rice, wheat, Brachypodium, Barley, Flax, and Phytophthora, visual screening of mutants, gene deletion (knock-out), tagging (knock-in) in mammalian cells, the cloning-free (DNA-free) technique, cell-penetrating peptides, generating a genome-edited banana, and nuclear genome editing of Chlamydomonas employing CRISPR-Cpf1 combined with a single-stranded DNA (ssODN) repair template. 

Authoritative and cutting-edge, CRISPR-Cas Methods aims to assist researchers who are newto the field and are aiming to learn how best to adopt this technology for a particular organism.

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Verlag
Humana
Erscheinungsdatum
2020
Sprache
Englisch
ISBN 10 
1071606158
ISBN 13 
9781071606155
Einband
Gebundene Ausgabe
Auflage
1
Anzahl der Seiten
292
Herausgeber
Islam M. Tofazzal, Bhowmik Pankaj K., Molla Kutubuddin A.
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