In Situ Hybridization Protocols (Methods in Molecular Biology, 123) - Hardcover

Inhaltsangabe

The technique of in situ hybridization, in its various forms, has been used routinely in many laboratories for a number of years. However, in situ hybridization has retained a reputation as one of the more difficult molecular biological techniques. This may be caused, in part, by the hybrid nature of the technique, which often requires a mixture of molecular biological and histological skills. The two techniques are usually taught and acquired in different streams of biolo- cal science. The step-by-step and detailed protocols provided in this book, by researchers active in the field, should make it possible for both the molecular biologist with little experience of histology and the histologist with little experience of molecular biology to use the technique successfully in their laboratories. In Situ Hybridization Protocols aims to provide useful protocols for two groups of researchers who use the technique: those who use in situ hybridi- tion to localize genes to particular chromosomes and those researchers who use DNA or RNA probes to localize expression of mRNA in tissue. This new edition also encompasses a number of techniques not covered in the previous edition, such as in situ hybridization of whole-mount embryo specimens, in situ hybridization at the electron microscopic level, and in situ detection of DNA fragmentation in apoptosis. The final two chapters of the book provide examples of application of in situ hybridization techniques to research and clinical problems.

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