Protocols for Neural Cell Culture - Softcover

Inhaltsangabe

In Protocols for Neural Cell Culture, 2nd Ed., Sergey Fedoroff and Arleen Richardson extensively revise and expand their best-selling and highly praised collection of popular neural tissue culture protocols. This second edition of unfailingly reproducible methods includes key techniques to study the development and differentiation of the nervous system, new methods for isolating and propagating progenitor cells that can be stimulated to differentiate into either neurons or various types of glial cells, and timely procedures for eliminating contaminating neural or nonneural cells. Important new procedures for the transfection of cells in culture with nontoxic mutant herpes simplex virus are also included, as are essential procedures for quantification, growth assays, evaluating neuroactive agents, preparing neural material for electron microscope analysis, and dissection.

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