Protein Targeting Protocols (Methods in Molecular Biology, Band 88) - Softcover

 
9780896034501: Protein Targeting Protocols (Methods in Molecular Biology, Band 88)
Softcover
ISBN 10: 089603450X ISBN 13: 9780896034501
Verlag: Humana, 1998

Inhaltsangabe

It is by no means a revelation that proteins are not uniformly distributed throughout the cell. As a result, the idea that protein molecules, because of the specificity with which they can engage in interactions with other proteins, may be aimed—via these interactions—at a restricted target, is a fundamental one in contemporary molecular life sciences. The target may be variously c- ceived as a specific molecule, a group of molecules, a structure, or a more generic type of intracellular environment. Because the concept of protein targeting is intuitive rather than expl- itly defined, it has been variously used by different groups of researchers in cell biology, biochemistry, and molecular biology. For those working in the field of intracellular signaling, an influential introduction to the topic was the seminal article by Hubbard & Cohen (TIBS [1993] 18, 172–177), which was based on the work of Cohen’s laboratory on protein phosphatases. Sub- quently, the ideas that they discussed have been further developed and extended by many workers to other key intermediaries in intracellular sign- ing, including protein kinases and a great variety of modulator and adaptor proteins.

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In Protein Targeting Protocols, master experimentalists describe, largely from the perspective of intracellular signaling, their best time-tested methods for detecting and analyzing the proteins and protein motifs involved in cellular targeting interactions. The techniques range from basic methods that reveal the existence of targeting interactions and characterize physicochemical properties to those for analyzing the more structurally elaborate systems involved in the movement of molecules between cellular compartments, with examples taken from nuclear transport, clathrin-coated vesicle traffic, and secretory sorting. The themes of targeting domains, particularly the SH-2, SH-3, and PH domains, and the principal targeting of lipid modifications-myristoylation, reversible palmitoylation, prenylation-are fully explored.

Protein Targeting Protocols offers a valuable collection of both basic and state-of-the-art techniques suitable for use in all areas of molecular cell biology by researchers interested in defining and characterizing the wide variety of protein interactions underlying targeting mechanisms. Readers will find it serves well as a firm methodological foundation for laboratories newly entering this field and as an indispensable reference work for those already well-established in it.




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Verlag
Humana
Erscheinungsdatum
1998
Sprache
Englisch
ISBN 10 
089603450X
ISBN 13 
9780896034501
Einband
Taschenbuch
Anzahl der Seiten
348
Herausgeber
Clegg Roger A.
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Weitere beliebte Ausgaben desselben Titels

9780896034877: Protein Targeting Protocols (Methods in Molecular Biology)

Vorgestellte Ausgabe

ISBN 10:  0896034879 ISBN 13:  9780896034877
Verlag: Humana Press, 1998
Hardcover